Difference between revisions of "Team:Dalhousie/Safety"

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</br>safety</br>
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On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)
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General Safety procedures:</br>
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Safe Project Design
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The Dalhousie iGEM team completed the biosafety training and WHIMIS prior to starting the
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Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:
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project. The biosafety and WHIMIS is an extensive biosafety online training that guides the
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Choosing a non-pathogenic chassis
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student through many fields of safety in the lab. The online modules addressed:</br>
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Choosing parts that will not harm humans / animals / plants
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- personal protective equipment (such as gloves, lab coats and safety goggles) in which
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Substituting safer materials for dangerous materials in a proof-of-concept experiment
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our team has been committed to</br>
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Including an "induced lethality" or "kill-switch" device
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- Chemical waste: all waste was autoclaved and disposed in its appropriate container by
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Safe Lab Work
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members of the Dalhousie iGEM.</br>
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What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!
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- Laboratory acquired infections:</br>
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Safe Shipment
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In addition to the general safety training, the Dalhousie iGEM laboratory was equipped with 2
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Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?
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fire extinguishers, a spill kit, eye wash stations, and a safety shower.</br>
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All Dalhousie iGEM members have successfully completed the online training and obtained
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their WHIMIS and biosafety training. The team also underwent subsequent laboratory training
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pertaining our project.</br></br>
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Project specific safety procedures:</br>
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The Dalhousie iGEM mentors conducted multiple dry workshops to go through project specific
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laboratory protocols with all members prior to conducting any experiments. After that, all
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members were trained for all protocols at the supervision of the mentors in the laboratory.</br>
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In addition, our project required some additional safety measures such as</br>
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- Operating a UV light box: Our project required the use of a UV light box to visualise the
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PCR product for cloning, therefore training on how to use the UV was essential to avoid
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burns and the over exposure that might lead to cancer.</br>
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- Bunsen burners: Aseptic techniques were required for sterile bacterial growth therefore
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members only turned on the gas of the Bunsen burners when necessary and turned off
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when done. All flammable materials were kept away when the Bunsen burner was on
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and personal protective equipment where worn at all times.</br>
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- Bacterial culture handling: Our project only used Escherichia coli in Risk Group 1 which
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are low risk bacteria that do not cause any disease in healthy individuals.</br>
  
  

Revision as of 00:58, 19 October 2017

Safety


General Safety procedures:
The Dalhousie iGEM team completed the biosafety training and WHIMIS prior to starting the project. The biosafety and WHIMIS is an extensive biosafety online training that guides the student through many fields of safety in the lab. The online modules addressed:
- personal protective equipment (such as gloves, lab coats and safety goggles) in which our team has been committed to
- Chemical waste: all waste was autoclaved and disposed in its appropriate container by members of the Dalhousie iGEM.
- Laboratory acquired infections:
In addition to the general safety training, the Dalhousie iGEM laboratory was equipped with 2 fire extinguishers, a spill kit, eye wash stations, and a safety shower.
All Dalhousie iGEM members have successfully completed the online training and obtained their WHIMIS and biosafety training. The team also underwent subsequent laboratory training pertaining our project.

Project specific safety procedures:
The Dalhousie iGEM mentors conducted multiple dry workshops to go through project specific laboratory protocols with all members prior to conducting any experiments. After that, all members were trained for all protocols at the supervision of the mentors in the laboratory.
In addition, our project required some additional safety measures such as
- Operating a UV light box: Our project required the use of a UV light box to visualise the PCR product for cloning, therefore training on how to use the UV was essential to avoid burns and the over exposure that might lead to cancer.
- Bunsen burners: Aseptic techniques were required for sterile bacterial growth therefore members only turned on the gas of the Bunsen burners when necessary and turned off when done. All flammable materials were kept away when the Bunsen burner was on and personal protective equipment where worn at all times.
- Bacterial culture handling: Our project only used Escherichia coli in Risk Group 1 which are low risk bacteria that do not cause any disease in healthy individuals.

Learn more... hopefully have links to next pages here