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Toehold switch structure: | Toehold switch structure: | ||
− | <p><center><img src="https://static.igem.org/mediawiki/2017/ | + | <p><center><img src="https://static.igem.org/mediawiki/2017/d/dc/CUHK_toeholdstructure.jpg" width="50%" height="auto" class=" igem-logo"></center></p> |
<p> | <p> | ||
− | We adopt the toehold switch design from the original paper. Our toehold switch contains 15nts “toehold domain”, 21nts stem | + | We adopt the toehold switch design from the original paper. Our toehold switch contains 15nts “toehold domain”, 21nts stem-loop that contains a start codon and a RBS B0034 loop at that toop. A 21nts linker and mRFP reporter sequence is present downstream the toehold switch. The linker is used to separate the coding sequence in the toehold switch and the reporter to prevent interference of protein folding. |
</p> | </p> | ||
</div> | </div> |
Revision as of 08:11, 22 October 2017