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     <p style="font-family: Poppins;font-size: 16px">今回用いた2つの融合シグナル伝達系によって、ヒト細胞と大腸菌のシグナル伝達を可能にした。これによって、細菌とほ乳類という界を超えたコミュニケーションが可能になり、iGEMの新しいstandardになると考えられる。</p>
 
     <p style="font-family: Poppins;font-size: 16px">今回用いた2つの融合シグナル伝達系によって、ヒト細胞と大腸菌のシグナル伝達を可能にした。これによって、細菌とほ乳類という界を超えたコミュニケーションが可能になり、iGEMの新しいstandardになると考えられる。</p>
 
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    <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px"><b>Discussion</b></h1>
 
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    <p style="font-family: Poppins;font-size: 16px">考察
 
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    <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px"><b>Reference</b></h1>
 
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    <p style="font-family: Poppins;font-size: 16px">参考文献
 
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<div class="w3-light-grey w3-container w3-padding-32" style="margin-top:75px;padding-right:58px"><p class="w3-right">Hajime Fujita:  <a href="96haji.me" title="W3.CSS" target="_blank" class="w3-hover-opacity">All Rights Reserved</a></p></div>
 
<div class="w3-light-grey w3-container w3-padding-32" style="margin-top:75px;padding-right:58px"><p class="w3-right">Hajime Fujita:  <a href="96haji.me" title="W3.CSS" target="_blank" class="w3-hover-opacity">All Rights Reserved</a></p></div>

Revision as of 09:59, 22 October 2017

<!DOCTYPE html> Coli Sapiens

iGEM Tokyo Tech

Achievements


Medal Criteria


Bronze Register and attend

We registered for iGEM, had a great summer, and attended the Giant Jamboree.

Deliverables

We met all deliverables on the Requirements page.

Attribution

We created a page on our team wiki with clear attribution of each aspect of our project.

Part

We documented 5 new BioBrick Parts (BBa_K1949100, BBa_K1949101, BBa_K1949102, BBa_K1949000, BBa_K1949001) central to our project and submitted them to the iGEM Registry .
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Silver Validated Part

We experimentally validated and documented 4 new BioBrick Parts/Devices (BBa_K1949050, BBa_K1949052, BBa_K1949030, BBa_K1949032) of our own design and construction that work as expected.
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Collaboration

We helped team KAIT_Japan model their system and taught a new high school team ASIJ about modeling.
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Human Practices

We considered our project from three aspects: education, ethics, and economy. To achieve this, we have done various outreach activities: School-visits, creating videos and a card game, hosting a symposium of bioethics, developing our original ethics code, and creating devices of protein production control. We also designed some genetic circuits which recreate other versions of Sow White. Through these activities, we could communicate with the public and improve our project from their feedback.
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Gold Integrated Human Practices

We held many activities in respect to our three aspects: education, ethics and economy. For example, we gave lessons to the students at 6 different high schools (269 students in total) and held a symposium. In addition, we applied the advice and ideas we got from the students and experts into our project. As a result, our Wet lab and Dry lab integrated to make "ACADwarfs," as well as figure out the concept of "TA system ~the Queen's Caprice~."
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Improvement / Characterization

We correctly improved a BioBrick Parts (BBa_R0071) and characterized 6 BioBrick Parts/Device (BBa_C0071, BBa_C0171, BBa_K1529300, Bba_K1529310, BBa_K1096002, BBa_K1096001)
Read more

Proof of concept

We demonstrated 4 functional proof of concept of our project: New cold inducible promoter, Rhl system assay, TA system, Degradation of C12 by AmiE.
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Best Information Processing


これまでのiGEMでは細菌同士の情報処理は盛んに行われてきた。今年我々はヒト細胞という細菌より複雑で、増殖速度などが細菌と大きく異なる細胞を対象にした。さらに両者を共培養することで、増殖速度や物質生産速度が大きく異なる細胞集団同士の情報処理に挑戦した。複雑系において重要な要素を選び出してモデルを抽象化し、シミュレーションした。結果、大腸菌数をヒト細胞によって制御し得ることや、共存可能なヒト細胞数と細菌数の関係を明らかにした。私たちはヒト細胞と大腸菌を共存させた新しい生命システムの構築(engineering)に成功した。これはiGEMの新たな一歩になると考えている。

Proof of Concept


今回用いた2つの融合シグナル伝達系によって、ヒト細胞と大腸菌のシグナル伝達を可能にした。これによって、細菌とほ乳類という界を超えたコミュニケーションが可能になり、iGEMの新しいstandardになると考えられる。

Hajime Fujita: All Rights Reserved