Difference between revisions of "Team:Kyoto/Experiments"
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<li><a href="https://2017.igem.org/team:Kyoto/Project">Project</a> | <li><a href="https://2017.igem.org/team:Kyoto/Project">Project</a> | ||
<ul> | <ul> | ||
| − | |||
<li><a href="https://2017.igem.org/Team:Kyoto/Results">Results</a></li> | <li><a href="https://2017.igem.org/Team:Kyoto/Results">Results</a></li> | ||
<li><a href="https://2017.igem.org/Team:Kyoto/Project_Details">Proof of concept</li> | <li><a href="https://2017.igem.org/Team:Kyoto/Project_Details">Proof of concept</li> | ||
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</ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Notebook">Notebook</a></li><li><a href="https://2017.igem.org/Team:Kyoto/integrated">Practice</a> | </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Notebook">Notebook</a></li><li><a href="https://2017.igem.org/Team:Kyoto/integrated">Practice</a> | ||
<ul> | <ul> | ||
| − | |||
<li><a href="https://2017.igem.org/Team:Kyoto/Collaborations">Collaboration</li> | <li><a href="https://2017.igem.org/Team:Kyoto/Collaborations">Collaboration</li> | ||
</ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Team">Team</a> | </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Team">Team</a> | ||
<ul> | <ul> | ||
| − | |||
<li><a href="https://2017.igem.org/Team:Kyoto/Attributions">Attributions</li> | <li><a href="https://2017.igem.org/Team:Kyoto/Attributions">Attributions</li> | ||
</ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Safety">Safety</a></li> | </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
</header> | </header> | ||
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<h1>Material&Methods</h1> | <h1>Material&Methods</h1> | ||
<ul class="material"> | <ul class="material"> | ||
Revision as of 11:36, 22 October 2017
Material&Methods
- 1.Parts
- 2.Primer list
- 3.Materials
- 3-1. Kit
- 3-2. Restricton Enzyme
- 3-3. Polymerase
- 3-4. DNA ligase
- 3-5. Marker
- 3-6. Organism
- 3-7. Antibiotics
- 3-8. Equipment
- 3-9. Backborns
- 3-10. Buffer
- 3-11. SDSPAGE/Western blotting
- 4.Methods
- 4-1. Miniprep
- 4-2. Gel Extraction
- 4-3. Restrict Enzyme Digestion
- 4-4. Ligation
- 4-5. Transformation
- 4-6. PCR
- 4-7. Sequencing
- 4-8. RT-PCR
- 4-10. Western blotting(Membrane fraction)
1.Parts
2.Primer list
| Primer name | Sequence | Length | Tm | GC% | Designer | Manufacturer |
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3.Materials
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