Difference between revisions of "Team:Cornell/Experiments"

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<h1>Experiments</h1>
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.  
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<h5>What should this page contain?</h5>
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<li> Protocols </li>
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<li> Experiments </li>
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<li> Documentation of the development of your project </li>
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<h5>Inspiration</h5>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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      <meta http-equiv="X-UA-Compatible" content="IE=edge">
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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      <title>Bios</title>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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      <meta name="description" content="Bios">
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              <li><a href="https://2017.igem.org/Team:Cornell/home">HOME</a></li>
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                      <li><a href="https://2017.igem.org/Team:Cornell/Parts">PARTS</a></li>
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                  <li><a href="#">PRODUCT DEVELOPMENT</a>
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                    <ul>
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                      <li><a href="https://2017.igem.org/Team:Cornell/productdevoverview">OVERVIEW</a></li>
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                      <li><a href="https://2017.igem.org/Team:Cornell/Hardware">OXYPONICS SYSTEM</a></li>
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                      <li><a href="https://2017.igem.org/Team:Cornell/Software">DASHBOARD</a></li>
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                      <li><a href="https://2017.igem.org/Team:Cornell/Design">DESIGN PROCESS</a></li>
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                    </ul>
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                  <li><a href="#">MATHEMATICAL MODELING</a>
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                    <ul>
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                      <li><a href="https://2017.igem.org/Team:Cornell/Model">MODELING</a></li>
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                      <li><a href="https://2017.igem.org/Team:Cornell/Animation">ANIMATION</a></li>
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                    </ul>
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              <li><a href="#">HUMAN CENTERED DESIGN</a>
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                  <li><a href="https://2017.igem.org/Team:Cornell/HP/Gold_Integrated">PRACTICES</a></li>
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                  <li><a href="https://2017.igem.org/Team:Cornell/Policies">POLICIES</a></li>
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                  <li><a href="https://2017.igem.org/Team:Cornell/Connections">CONNECTIONS</a></li>
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                  <li><a href="">OFFICIAL PROFILE</a></li>
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                    <li><a href="#overview">OVERVIEW</a></li>
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                    <li><a href="#biobricks">BIOBRICKS</a></li>
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                    <li><a href="#chassis">CHASSIS</a></li>
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                    <li><a href="#results">RESULTS</a></li>
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                    <li><a href="#futurework">FUTURE WORK</a></li>
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                    <li><a href="#references">REFERENCES</a></li>
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                      <div class="standard-title"><a id="overview">Overview</a></div>
 +
                          <p>Oxyponics was conceived in the summer of 2017 by Cornell iGEM -  a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.
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                          </p>
 +
                      <div class="standard-title"><a id="biobricks">Biobricks</a></div>
 +
                        <p>Oxyponics was conceived in the summer of 2017 by Cornell iGEM -  a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.
 +
                        </p>
 +
                      <div class="standard-title"><a id="chassis">Chassis</a></div>
 +
                        <p>Oxyponics was conceived in the summer of 2017 by Cornell iGEM -  a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.
 +
                        </p>
 +
                      <div class="standard-title"><a id="results">Results</a></div>
 +
                        <p>Oxyponics was conceived in the summer of 2017 by Cornell iGEM -  a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.
 +
                        </p>
 +
                      <div class="standard-title"><a id="futurework"> Future Work</a></div>
 +
                        <p>Oxyponics was conceived in the summer of 2017 by Cornell iGEM -  a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.
 +
                        </p>
 +
                      <div class="standard-title"><a id="references">References</a></div>
 +
                        <p>Oxyponics was conceived in the summer of 2017 by Cornell iGEM -  a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.
 +
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Revision as of 00:54, 23 October 2017

<!DOCTYPE html> Bios

Overview

Oxyponics was conceived in the summer of 2017 by Cornell iGEM - a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.

Biobricks

Oxyponics was conceived in the summer of 2017 by Cornell iGEM - a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.

Chassis

Oxyponics was conceived in the summer of 2017 by Cornell iGEM - a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.

Results

Oxyponics was conceived in the summer of 2017 by Cornell iGEM - a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.

Future Work

Oxyponics was conceived in the summer of 2017 by Cornell iGEM - a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.

References

Oxyponics was conceived in the summer of 2017 by Cornell iGEM - a synthetic biology engineering project team composed of 36 undergraduate students. The company created a toolkit to optimize redox state by controlling the amount of reactive oxygen species in the environment. Utilizing the research conducted by the team throughout 2017, Oxyponics created a novel biosensor that couples a redox-sensitive fluorescent protein and an optogenetic circuit to a spectrometric receiver. This allows for a dual reporter-response functionality that greatly enhances versatility and sensitivity over traditional biosensor systems. With the development of an effective large-scale sensing system to monitor plants and track data, the optimal level of oxidative stress that can be determined for the plant systems. Members of the team communicated with local farmers to learn more about Through the feedback of farmers themselves, we created Oxyponics, which couples fluorescent bacteria with a camera-rail system for imaging, to provide exactly the large-scale blanket-type sensing that conventional redox probes cannot.