Difference between revisions of "Team:BNU-China/Results"
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<h1>Results</h1> | <h1>Results</h1> | ||
<h2 id="title1">Microtubule</h2> | <h2 id="title1">Microtubule</h2> | ||
| − | <h3>Plasmid construction</h3> | + | <h3>Plasmid construction<br></h3> |
| − | <p>We have accomplished the construction of 6 main parts whose functions are described respectively in previous design page. <a href="https://2017.igem.org/Team:BNU-China/Design#title2">( | + | <p>We have accomplished the construction of 6 main parts whose functions are described respectively in previous design page. <a href="https://2017.igem.org/Team:BNU-China/Design#title2">(Microtubule module)</a></p> |
<img src="https://static.igem.org/mediawiki/2017/e/e1/T-BNU-China-results1.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/e/e1/T-BNU-China-results1.png" alt="Sorry, the image is not spupported by your browser."> | ||
<h4>Figure 1 The electrophoresis image of these 6 plasmids.</h4> | <h4>Figure 1 The electrophoresis image of these 6 plasmids.</h4> | ||
| − | <h3>Protein expression</h3> | + | <h3>Protein expression<br></h3> |
<p>These are images recorded by the Fluorescence microscope.The engineered yeasts were induced at 30°C for 20 hours. And from the image, we can notice that our engineered yeasts have successfully expressed our recombinant proteins mCherry-αand mCherry. Moreover, the expression rate of mCherry is almost up to 100%. </p> | <p>These are images recorded by the Fluorescence microscope.The engineered yeasts were induced at 30°C for 20 hours. And from the image, we can notice that our engineered yeasts have successfully expressed our recombinant proteins mCherry-αand mCherry. Moreover, the expression rate of mCherry is almost up to 100%. </p> | ||
<img src="https://static.igem.org/mediawiki/2017/1/19/T-BNU-China-results2.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/1/19/T-BNU-China-results2.png" alt="Sorry, the image is not spupported by your browser."> | ||
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The target proteins are expressed by the engineered yeasts which contain pYCα-alpha, pYCα-beta, pYCα-mCherry and pYCα-mCherry-αtubulin, respectively.</p> | The target proteins are expressed by the engineered yeasts which contain pYCα-alpha, pYCα-beta, pYCα-mCherry and pYCα-mCherry-αtubulin, respectively.</p> | ||
<img src="https://static.igem.org/mediawiki/2017/2/25/T-BNU-China-results3.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/2/25/T-BNU-China-results3.png" alt="Sorry, the image is not spupported by your browser."> | ||
| − | <h4>Figure 3 The results of a Western blot analysis carried out with an anti-V5 antibody</h4> | + | <h4>Figure 3 The results of a Western blot analysis carried out with an anti-V5 antibody.</h4> |
| − | <p>Our engineered yeasts, containing pYD1-β tubulin vector, have been proved | + | <p>Our engineered yeasts, containing pYD1-β tubulin vector, have been proved successfully expressing exogenous βtubulin by Western blot analysis.</p> |
<img src="https://static.igem.org/mediawiki/2017/9/9f/T-BNU-China-results4.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/9/9f/T-BNU-China-results4.png" alt="Sorry, the image is not spupported by your browser."> | ||
| − | <h4>Figure 4 The partly results of a Western blot analysis carried out with an anti-V5 antibody</h4> | + | <h4>Figure 4 The partly results of a Western blot analysis carried out with an anti-V5 antibody.</h4> |
<h2 id="title2">Flagellar Filament</h2> | <h2 id="title2">Flagellar Filament</h2> | ||
| − | <h3>Plasmid construction</h3> | + | <h3>Plasmid construction<br></h3> |
| − | <h3>Protein expression</h3> | + | <p>We have successfully constructed the following 11 parts that have been described in detail in previous design page. <a href="https://2017.igem.org/Team:BNU-China/Design#title3">(Filagellar filament module)</a> These are the electrophoresis image of our parts. </p> |
| + | |||
| + | <h3>Protein expression<br></h3> | ||
<img src="https://static.igem.org/mediawiki/2017/e/e7/T--BNU-China--results5.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/e/e7/T--BNU-China--results5.png" alt="Sorry, the image is not spupported by your browser."> | ||
<h4>figure 5</h4> | <h4>figure 5</h4> | ||
Revision as of 02:25, 23 October 2017
Results
Microtubule
Plasmid construction
We have accomplished the construction of 6 main parts whose functions are described respectively in previous design page. (Microtubule module)
Figure 1 The electrophoresis image of these 6 plasmids.
Protein expression
These are images recorded by the Fluorescence microscope.The engineered yeasts were induced at 30°C for 20 hours. And from the image, we can notice that our engineered yeasts have successfully expressed our recombinant proteins mCherry-αand mCherry. Moreover, the expression rate of mCherry is almost up to 100%.
Figure 2 Induced 20h in SG-Ura medium；A,B: recipient strain with empty plasmid
The image shows the results of a Western blot analysis carried out with an anti-V5 antibody. The target proteins are expressed by the engineered yeasts which contain pYCα-alpha, pYCα-beta, pYCα-mCherry and pYCα-mCherry-αtubulin, respectively.
Figure 3 The results of a Western blot analysis carried out with an anti-V5 antibody.
Our engineered yeasts, containing pYD1-β tubulin vector, have been proved successfully expressing exogenous βtubulin by Western blot analysis.
Figure 4 The partly results of a Western blot analysis carried out with an anti-V5 antibody.
Flagellar Filament
Plasmid construction
We have successfully constructed the following 11 parts that have been described in detail in previous design page. (Filagellar filament module) These are the electrophoresis image of our parts.
Protein expression
figure 5
figure 6
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