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− | < | + | <b> <center><p style="font-family: quicksand;font-size:200%;">RNA thermodynamic modeling: Designing Toehold Switch </p></center> </b> |
− | < | + | <p style="font-family: quicksand;font-size:130%;">Background </p> |
− | <p> | + | <p> <h4> |
− | According to Green et al., the optimal length of RNA to be detected by a toehold switch is around 30 bp. In other words, a target RNA with 1000 bp in length can have 970 possible switches. However, the performances of each possible switch will be different, since switches that target different region will have different thermodynamic characteristic and structure, which can affect the performance of the switch. Therefore, we modeled the thermodynamic and structure of our toehold switch during designing stage and simulate the expression of activated switch in silico. Our modelling helped us a lot in gaining insight. | + | According to Green et al., the optimal length of RNA to be detected by a toehold switch is around 30 bp. In other words, a target RNA with 1000 bp in length can have 970 possible switches. However, the performances of each possible switch will be different, since switches that target different region will have different thermodynamic characteristic and structure, which can affect the performance of the switch. Therefore, we modeled the thermodynamic and structure of our toehold switch during designing stage and simulate the expression of activated switch in silico. Our modelling helped us a lot in gaining insight. </h4> |
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Revision as of 12:30, 24 October 2017