Line 6: | Line 6: | ||
<h2>Overview</h2> | <h2>Overview</h2> | ||
+ | <p>After the E. coli BL21 (DE3) engineered by the Synthesis subgroup has produced polyhydroxybutyrate (PHB), it is stored as intracellular granules that range in size from 60-80nm. This creates a challenging problem: efficient extraction of the desired PHB from inside the cell. During the early stages of our project, we explored many possible PHB extraction methods.</p> | ||
+ | |||
<h2>Type 1 Secretion</h2> | <h2>Type 1 Secretion</h2> | ||
<h2>Our Secretion System</h2> | <h2>Our Secretion System</h2> |
Revision as of 04:29, 26 October 2017
Secretion
Overview
After the E. coli BL21 (DE3) engineered by the Synthesis subgroup has produced polyhydroxybutyrate (PHB), it is stored as intracellular granules that range in size from 60-80nm. This creates a challenging problem: efficient extraction of the desired PHB from inside the cell. During the early stages of our project, we explored many possible PHB extraction methods.
Type 1 Secretion
Our Secretion System
Our Construct
Experiments
Future Directions
Works Cited