Difference between revisions of "Team:ETH Zurich/Protocols"
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<summary>Chemical Transformation</summary> | <summary>Chemical Transformation</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>Colony PCR</summary> | <summary>Colony PCR</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>Consumables</summary> | <summary>Consumables</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>Cryostock of Bacteria</summary> | <summary>Cryostock of Bacteria</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>Electroporation</summary> | <summary>Electroporation</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>Gel Extraction</summary> | <summary>Gel Extraction</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>Gibson Assembly</summary> | <summary>Gibson Assembly</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>Ligase Chain Reaction</summary> | <summary>Ligase Chain Reaction</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>Miniprep</summary> | <summary>Miniprep</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>PCR Clean-Up</summary> | <summary>PCR Clean-Up</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" width="100%" height="700px"/> |
</details> | </details> | ||
<details> | <details> | ||
<summary>Phusion PCR</summary> | <summary>Phusion PCR</summary> | ||
| − | <embed src="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" width="100%" height=" | + | <embed src="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" width="100%" height="700px"/> |
| + | </details> | ||
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| + | <details> | ||
| + | <summary>Preparation of Chemically Competent Cells</summary> | ||
| + | <embed src="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" width="100%" height="700px"/> | ||
| + | </details> | ||
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| + | <details> | ||
| + | <summary>Preparation of Electrocompetent Cells</summary> | ||
| + | <embed src="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" width="100%" height="700px"/> | ||
| + | </details> | ||
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| + | <details> | ||
| + | <summary>Restriction Ligation</summary> | ||
| + | <embed src="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" width="100%" height="700px"/> | ||
</details> | </details> | ||
Revision as of 11:52, 26 October 2017
Protocols
General Protocols
Chemical Transformation
Colony PCR
Consumables
Cryostock of Bacteria
Electroporation
Gel Extraction
Gibson Assembly
Ligase Chain Reaction
Miniprep
PCR Clean-Up
Phusion PCR
Preparation of Chemically Competent Cells
Preparation of Electrocompetent Cells
Restriction Ligation
