Difference between revisions of "Team:ETH Zurich/Protocols"

Line 116: Line 116:
 
<section>
 
<section>
 
<h1>Heat Sensor</h1>
 
<h1>Heat Sensor</h1>
 +
<details>
 +
        <summary>Heat-Induction of TlpA-Repressed GFP Expression</summary>
 +
        <embed src="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" width="100%" height="700px"/>
 +
</details>
 
</section>
 
</section>
  

Revision as of 12:14, 26 October 2017

Protocols

General Protocols

Chemical Transformation
Colony PCR
Consumables
Cryostock of Bacteria
Electroporation
Gel Extraction
Gibson Assembly
Ligase Chain Reaction
Miniprep
PCR Clean-Up
Phusion PCR
Preparation of Chemically Competent Cells
Preparation of Electrocompetent Cells
Restriction Ligation

Tumor Sensor

MRI Contrast Agent

Fluorescence Measurement to Obtain the AHL Dose-Response Curve
SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin
Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle

Anti-Cancer Toxin

Heat Sensor

Heat-Induction of TlpA-Repressed GFP Expression

Cell Lysis