Difference between revisions of "Team:Munich/Hardwarevalve"

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Our pathogen detection approach relies on Cas13a digesting RNA. A common way of monitoring RNase activities is using commercially available RNaseAlert, consisting of a fluorescent RNA beacon. This is impractical for in-field applications because commercial fluorescence detectors are expensive and inconveniently large. We therefore make our pathogen detection system fit for in-field applications by developing a cheap and handy fluorescence detector. Although many previous iGEM teams constructed fluorescence detectors, we could not find any that had a high enough sensitivity or the ability to measure fluorescence quantitatively. We therefore constructed a detector matching our requirements and compared it to others in a cost vs sensitivity diagram.
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To control fluid flow in our final device we constructed pneumatic controlled water valves. We use electric powered air valves to control these so-called quake valves and build a circuit to control the air valves with a microcontroller. This enables us to precisely move fluids on our final device via a software interface. We constructed macroscopic quake valves with 3D printed negatives out of PDMS via soft lithography. The valves can be scaled down and are require now special equipment for their manufacture. A detailed protocol for manufacturing macroscopic fluidic chips with 3D Printed negatives can be found <a class="myLink" href="https://2017.igem.org/Team:Munich/Protocols">here</a> at the subitem "Soft lithography"
 
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Revision as of 14:25, 26 October 2017

Description

Design

Demonstrate

Measurement

Applied Design

Final Results

Achievements

Protocols

Notebook

Safety

Parts

Improved Part

Part Collection

InterLab

Model

Software

Collaboration

Detector

Quake Valve

Paperstrips

Sample Processing

Silver

Gold

Engagement

Collaborations

Team members

Sponsors

Attributions

Gallery

Hardware

To control fluid flow in our final device we constructed pneumatic controlled water valves. We use electric powered air valves to control these so-called quake valves and build a circuit to control the air valves with a microcontroller. This enables us to precisely move fluids on our final device via a software interface. We constructed macroscopic quake valves with 3D printed negatives out of PDMS via soft lithography. The valves can be scaled down and are require now special equipment for their manufacture. A detailed protocol for manufacturing macroscopic fluidic chips with 3D Printed negatives can be found here at the subitem "Soft lithography"