Difference between revisions of "Team:Munich/Hardwarevalve"

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<font size=7 color=#51a7f9><b style="color: #51a7f9">Hardware</b></font>
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<font size=7 color=#51a7f9><b style="color: #51a7f9">Electric Controlled Quake Valves</b></font>
 
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<p class="introduction">
 
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Our pathogen detection approach relies on Cas13a digesting RNA. A common way of monitoring RNase activities is using commercially available RNaseAlert, consisting of a fluorescent RNA beacon. This is impractical for in-field applications because commercial fluorescence detectors are expensive and inconveniently large. We therefore make our pathogen detection system fit for in-field applications by developing a cheap and handy fluorescence detector. Although many previous iGEM teams constructed fluorescence detectors, we could not find any that had a high enough sensitivity or the ability to measure fluorescence quantitatively. We therefore constructed a detector matching our requirements and compared it to others in a cost vs sensitivity diagram.
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To control fluid flow in our final device we constructed pneumatic controlled water valves. We use electric powered air valves to control these so-called quake valves and build a circuit to control the air valves with a microcontroller. This enables us to precisely move fluids on our final device via a software interface. We constructed macroscopic quake valves with 3D printed negatives out of PDMS via soft lithography. The valves can be scaled down and are require now special equipment for their manufacture. A detailed protocol for manufacturing macroscopic fluidic chips with 3D Printed negatives can be found <a class="myLink" href="https://2017.igem.org/Team:Munich/Protocols">here</a> at the subitem "Soft lithography".
 
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The valve is made of three PDMS layers. The lower layer carries the water channel with a sinus shaped dome at the valve position. The middle layer is just a thin and therefore elastic PDMS film. The upper layer carries the air channel which as a cylindric air reservoir at the valve position. The three layers are shown in the explosive drawing below.
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<img src="https://static.igem.org/mediawiki/2017/d/d2/T--Munich--Hardware_explodevalve.svg">
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Explosive drawing of a quake valve.
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If air pressure is applied to the upper channel the middle layer will be pressed in the sinus shaped dome of the upper channel and block the water channel. We chose a sinus shape for the lower channel to enable a smooth contact between the thin layer and lower layer. This mechanism is illustrated in the figure below
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<img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Hardware_sketchvalve.svg">
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Function of a quake valve.
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The Air channel is connected via tubes to two normally closed electric air valves. One to release pressure for opening the water valve and one to apply new pressure for closing the water valve.
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<p>
  
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We need a control circuit for the air valves because they are powered with high currents and consist of an electric coil that causes voltage pikes which can be harmful to a microcontroller
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The electric valve is connected to the drain of drain of a N Channel MOSFET. The drain is connected via a voltage divider to a digital pin. When the digital Pin is set to 5V, the voltage at the gate is above the threshold voltage, current flows through the Valve and the air channel is opened. A diode in blocking direction parallel to valve damps the voltage spike caused by turning of the valve. The circuit is shown in the figure below.
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<div class="captionPicture">
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<img width = 500 src="https://static.igem.org/mediawiki/2017/6/60/T--Munich--Hardware_valvecircuit.svg">
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Control circuit for the electric valves.
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Latest revision as of 15:28, 26 October 2017

Description

Design

Demonstrate

Measurement

Applied Design

Final Results

Achievements

Protocols

Notebook

Safety

Parts

Improved Part

Part Collection

InterLab

Model

Software

Collaboration

Detector

Quake Valve

Paperstrips

Sample Processing

Silver

Gold

Engagement

Collaborations

Team members

Sponsors

Attributions

Gallery

Electric Controlled Quake Valves

To control fluid flow in our final device we constructed pneumatic controlled water valves. We use electric powered air valves to control these so-called quake valves and build a circuit to control the air valves with a microcontroller. This enables us to precisely move fluids on our final device via a software interface. We constructed macroscopic quake valves with 3D printed negatives out of PDMS via soft lithography. The valves can be scaled down and are require now special equipment for their manufacture. A detailed protocol for manufacturing macroscopic fluidic chips with 3D Printed negatives can be found here at the subitem "Soft lithography".

The valve is made of three PDMS layers. The lower layer carries the water channel with a sinus shaped dome at the valve position. The middle layer is just a thin and therefore elastic PDMS film. The upper layer carries the air channel which as a cylindric air reservoir at the valve position. The three layers are shown in the explosive drawing below.

Explosive drawing of a quake valve.

If air pressure is applied to the upper channel the middle layer will be pressed in the sinus shaped dome of the upper channel and block the water channel. We chose a sinus shape for the lower channel to enable a smooth contact between the thin layer and lower layer. This mechanism is illustrated in the figure below

Function of a quake valve.

The Air channel is connected via tubes to two normally closed electric air valves. One to release pressure for opening the water valve and one to apply new pressure for closing the water valve.

We need a control circuit for the air valves because they are powered with high currents and consist of an electric coil that causes voltage pikes which can be harmful to a microcontroller The electric valve is connected to the drain of drain of a N Channel MOSFET. The drain is connected via a voltage divider to a digital pin. When the digital Pin is set to 5V, the voltage at the gate is above the threshold voltage, current flows through the Valve and the air channel is opened. A diode in blocking direction parallel to valve damps the voltage spike caused by turning of the valve. The circuit is shown in the figure below.

Control circuit for the electric valves.