Difference between revisions of "Team:ETH Zurich/Protocols"

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<h1>General Protocols</h1>
 
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         <summary>Chemical Transformation</summary>
 
         <summary>Chemical Transformation</summary>
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<h1>Cell Lysis</h1>
 
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Revision as of 05:16, 27 October 2017

Protocols

General Protocols

Chemical Transformation
Colony PCR
Consumables
Cryostock of Bacteria
Electroporation
Gel Extraction
Gibson Assembly
Ligase Chain Reaction
Miniprep
PCR Clean-Up
Phusion PCR
Preparation of Chemically Competent Cells
Preparation of Electrocompetent Cells
Restriction Ligation

Tumor Sensor

MRI Contrast Agent

Fluorescence Measurement to Obtain the AHL Dose-Response Curve
SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin
Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle

Anti-Cancer Toxin

Fluorescence Measurement to Obtain the AHL Dose-Response Curve
SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28

Heat Sensor

Heat-Induction of TlpA-Repressed GFP Expression

Cell Lysis