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| − | <title>Team:Shenzhen SFLS/Parts - 2017.igem.org</title>
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| + | <h1>Parts</h1> |
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| | + | <div class="column half_size"> |
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| | + | <h5>Note</h5> |
| | + | <p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p> |
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| − | <h1>Parts</h1>
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| − | <a class="taoba" href="#index_2" title="2">Part Table</a>
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| − | <!-- <a class="taoba" href="#index_3" title="3">The future plan </a>
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| − | <a class="taoba" href="#index_4" title="4">Reference</a> -->
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| + | <h5>Adding parts to the registry</h5> |
| | + | <p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p> |
| | + | <p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p> |
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| − | <h2 class="">PREVENTION: GSR FUNCTION</h2>
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| − | <h5 id="index_1">The effects of the CRISPR/Cas9 system on the proliferation, migration and apoptosis in melanoma cells.
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| − | </h5>
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| − | <h6>Cell proliferation was inhibited by the CRISPR/Cas9 system in melanoma cells.</h6>
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| − | <p>The CCK-8 assay was used to investigate the effects of the CRISPR/Cas9 system on cell growth in melanoma cells. Compared with the negative control, cell growth was significantly inhibited in A375 cells (Fig.4A) and G361 cells (Fig.4B) after
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| − | treatment of the CRISPR/Cas9 system. </p>
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| − | <img src="https://static.igem.org/mediawiki/2017/f/fb/Sfls-demonstrate_fig.1.PNG" width="700" alt="demonstrate_fig.1">
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| − | <br/><br/>
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| − | <h6>Cell migration was suppressed by CRISPR/Cas9 system in melanoma cells. </h6>
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| − | <p>The transwell assay was performed to detect the effects of these devices on cell migration. Compared with the NC group, the cell migration rate was obviously lower in the Treatment group both in A375 and G361 cells (Fig 2. A-D). </p>
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| − | <img src="https://static.igem.org/mediawiki/2017/3/3c/Sfls-demonstrate_fig.2.PNG" width="700" alt="demonstrate_fig.2">
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| − | <br/><br/>
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| − | <h6>Cell apoptosis was significantly promoted by the CRISPR/Cas9 system in melanoma cells. </h6>
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| − | <p>Flow cytometry was performed to detect the effects of the CRISPR/Cas9 system on cell apoptosis. As shown in Fig. 6A-D, compared with the negative control group, cell apoptosis was promoted significantly in A375 and G361 cells in the Treatment
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| − | group. The results show that cell apoptosis was inhibited significantly by the synthetic system in melanoma cells. </p>
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| | + | <div class="column half_size"> |
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| + | <h5>What information do I need to start putting my parts on the Registry?</h5> |
| − | <img src="https://static.igem.org/mediawiki/2017/c/cd/Sfls-demonstrate_fig.3.PNG" width="700" alt="demonstrate_fig.3">
| + | <p>The information needed to initially create a part on the Registry is:</p> |
| − | </div>
| + | <ul> |
| − | <br/><br/>
| + | <li>Part Name</li> |
| | + | <li>Part type</li> |
| | + | <li>Creator</li> |
| | + | <li>Sequence</li> |
| | + | <li>Short Description (60 characters on what the DNA does)</li> |
| | + | <li>Long Description (Longer description of what the DNA does)</li> |
| | + | <li>Design considerations</li> |
| | + | </ul> |
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| − | </div>
| + | <p> |
| | + | We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p> |
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| + | <h5>Inspiration</h5> |
| | + | <p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p> |
| | | | |
| | + | <p> You can also take a look at how other teams have documented their parts in their wiki:</p> |
| | + | <ul> |
| | + | <li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li> |
| | + | <li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li> |
| | + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li> |
| | + | </ul> |
| | + | </div> |
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| − | <div class=" mainpage mainpage2"> | + | <div class="column full_size"> |
| − | <h5 id="index_2" style="color:#fc3842 !important">Part Table</h5>
| + | <h5>Part Table </h5> |
| − | <table id="table">
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| + | <p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p> |
| − | <th><b>Name</b></th>
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| − | <th><b>Type</b></th>
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| − | <th><b>Description</b></th>
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| − | <th><b>Length</b></th>
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| + | <groupparts>iGEM17 Shenzhen_SFLS</groupparts> |
| − | <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2200000'>BBa_K2200000</a></td>
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| − | <td>RNA</td>
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| − | <td>Guide RNA (gRNA) is a specific molecule that guides the Cas nuclease to a targeted dsDNA sequence.</td>
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| − | <td>20</td>
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| − | <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2200001'>BBa_K2200001</a></td>
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| − | <td>RNA</td>
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| − | <td>gRNA used as a control</td>
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| − | <td>20</td>
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| − | <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2200003'>BBa_K2200003</a></td>
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| − | <td>Regulatory</td>
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| − | <td>Human U6 promoter is a common part for eukaryotic expression systems.</td>
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| − | <td>258</td>
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| − | </tr>
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| − | <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2200004'>BBa_K2200004</a></td>
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| − | <td>Regulatory</td>
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| − | <td>pHEf1A promoter is a common promoter for eukaryotic expression systems.</td>
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| − | <td>1174</td>
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| − | </tr>
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| − | <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2200005'>BBa_K2200005</a></td>
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| − | <td>Coding</td>
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| − | <td>hCas9</td>
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| − | <td>4104</td>
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| − | <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2200007'>BBa_K2200007</a></td>
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| − | <td>Coding</td>
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| − | <td>Gal4 transcription factor is a positive regulator.It's activity can be greatly enhanced by adding th</td>
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| − | <td>1233</td>
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| − | <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2200008'>BBa_K2200008</a></td>
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| − | <td>Regulatory</td>
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| − | <td>pHEf1A promoter is a common promoter for eukaryotic expression systems.</td>
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| − | <td>1174</td>
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| − | </tr>
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| − | <h5 id="index_3">The future plan </h5>
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| − | <p>In the future, we would like to verify the system if it can work as expected. Then we would put the regulatory system and CRISPR/Cas9 system together and measure the growth of normal cells and melanoma cells to see whether Cas9 protein only
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| − | expresses in melanoma cells. </p>
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| − | <p>Next, we will pack the vectors containing the amiRNA, amiRNA binding-site, Cas9 and sgRNA into lentivirus in order to increase the transfection efficiency and use them to deliver the constructs into the target cells in the functional experiments.
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| − | </p>
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| − | <p>Finally, we also want to sequence the whole genome of the transfected cell and see whether we can reduce the off-target effects on the melanoma cells by the system. </p>
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| − | <h5 id="index_4">Reference</h5>
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| − | <li>Koo T, Yoon A R, Cho H Y, et al. Selective disruption of an oncogenic mutant allele by CRISPR/Cas9 induces efficient tumor regression[J]. Nucleic Acids Research, 2017.</li>
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| − | <li>Huang X, Zhuang C, Zhuang C, et al. An enhanced hTERT promoter-driven CRISPR/Cas9 system selectively inhibits the progression of bladder cancer cells.[J]. Molecular Biosystems, 2017.</li>
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| − | <h3><a href="home.html">SFLS.</a></h3>
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| − | <p id="ours">Shenzhen Foreign Language School, Yantian Road No.1, Yantian District, Shenzhen, Guangdong, China</p>
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| − | <li><a href="https://2017.igem.org/Team:Shenzhen_SFLS/Description ">Description</a></li>
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| − | <li><a href="https://2017.igem.org/Team:Shenzhen_SFLS/Design ">Design</a></li>
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| − | <li><a href="https://2017.igem.org/Team:Shenzhen_SFLS/Experiments ">Experiment</a></li>
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| − | <li><a href="https://2017.igem.org/Team:Shenzhen_SFLS/Basic_Part ">Basic Part</a></li>
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| − | <li><a href="https://2017.igem.org/Team:Shenzhen_SFLS/Composite_Part ">Composite Part</a></li>
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| − | <li><a href="https://2017.igem.org/Team:Shenzhen_SFLS/Model ">Modeling</a></li>
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| − | <li><a href="https://2017.igem.org/Team:Shenzhen_SFLS/HP/Silver ">HP-Silver</a></li>
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