Material&Methods

• 1.Parts
• 2.Primer list
• 3.Materials
• 3-1. Kit
• 3-2. Restricton Enzyme
• 3-3. Polymerase
• 3-4. DNA ligase
• 3-5. Marker
• 3-6. Organism
• 3-7. Antibiotics
• 3-8. Equipment
• 3-9. Backborns
• 3-10. Buffer
• 3-11. SDSPAGE/Western blotting
• 4.Methods
• 4-1. Miniprep
• 4-2. Gel Extraction
• 4-3. Restrict Enzyme Digestion
• 4-4. Ligation
• 4-5. Transformation
• 4-6. PCR
• 4-7. Sequencing
• 4-8. RT-PCR
• 4-10. Western blotting(Membrane fraction)

1.Parts

2.Primer list

Primer name	Sequence	Length	Tm	GC%	Designer	Manufacturer

3.Materials

3-1.Kit

Name	Supplier

3-2.Restriction Enzyme

Name	Supplier

3-3.Polymerase

Name	Supplier

3-4.DNA Ligase

Name	Supplier

3-5.Marker

Name	Supplier

3-6.Organism

Name	Supplier

3-7.Antibiotics

Name	Supplier

3-8.Equipment

Name	Supplier

3-9.Backbones

Name	Supplier

3-10.Buffer

Name	Supplier

3-11.SDSPAGE/Western blotting

Name	Supplier

4.Methods

4-1.Miniprep

4-2.Gel Extraction

4-3.Restriction Enzyme Digestion

4-4.Ligation

4-5.Transformation

4-6.PCR

4-7.Sequenicing

4-8.RT-PCR

4-9.Basic protcol of Western blotting

4-10.Western blotting(Membrane fraction)