Difference between revisions of "Team:Cadets2Vets/Design"

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Our circuit was designed to utilize specific parts so that when the E-coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.&nbsp;</font><div><br></div></h3></div></div></div><div class="sDivider col-md-6 index1" data-colsize="6" data-title="Column"><div class="simblaEL simblaImg" data-drag="P20" id="P20"><img draggable="false" class="" alt="" data-id="" title="" src="https://s3.amazonaws.com/simbla-static/2017/5/593aca5737cad4ed7fa1fc16/12707-ymd99p.jpg"></div></div></div></div></div></div></div><div class="simblaEL containerHolder" data-drag="P113" id="P113" style="position: relative;"><a class="simblaEL anchorElm" data-drag="divCounter" name="Content1" id="Content1" data-id="5941796e95f86d9166bcfe6c"></a><div class="container-fluid" style="border-radius: 0px; padding-top: 50px; padding-bottom: 50px;" data-border-type="All" data-title="Container"><div class="row simblaEL rDivider" data-drag="P115" id="P115" style="position: relative;" data-title="Row"><div class="sDivider col-md-4 index0" data-colsize="4" data-title="Column"><div class="simblaEL simblaGallery" data-drag="P117" id="P117" data-g-hover-color="" data-g-text-color="" data-g-style="FADE" data-g-spacing="NORMAL"><div class="row simblaEL rDivider" data-title="Row"><div class="sDivider elem col-md-12 col-sm-12" data-title="Column"></div></div></div><div class="simblaEL tcH" data-drag="P121" id="P121" style="position: relative;"><div class="textContainer H2" style="margin-left: 10px; cursor: text;"><h2>PHASE 1</h2></div></div><div class="simblaEL tc" data-drag="P123" id="P123" style="position: relative;"><div class="textContainer" style="cursor: text; margin-left: 10px; line-height: 24px;"><p style="line-height: 24px;"></p><ul><li><font style="font-size: 18px;">Test efficacy of the circuit in vivo&nbsp;<br></font></li><li><font style="font-size: 18px;">Grow transformed e.coli with plasma circuit in Arsenic laced media &nbsp;&nbsp;</font></li><li><font style="font-size: 18px;">Serial dilution of arsenic and arsenic to find the lowest activation amount of the circuit in vivo</font><br></li></ul><p></p></div></div></div><div class="sDivider col-md-4 index1" data-colsize="4" data-title="Column"><div class="simblaEL simblaGallery" data-drag="P127" id="P127" data-g-hover-color="" data-g-text-color="" data-g-style="FADE" data-g-spacing="NORMAL"><div class="row simblaEL rDivider" data-title="Row"><div class="sDivider elem col-md-12 col-sm-12" data-title="Column"></div></div></div><div class="simblaEL tcH" data-drag="P131" id="P131" style="position: relative;"><div class="textContainer H2" style="margin-left: 10px; cursor: text;"><h2>PHASE 2</h2></div></div><div class="simblaEL tc" data-drag="P133" id="P133" style="position: relative;"><div class="textContainer" style="cursor: text; margin-left: 10px; line-height: 24px;"><p style="line-height: 24px;"></p><ul><li><font style="font-size: 18px;">Test the efficacy of the plasma circuit in S.I.T.U. &nbsp;&nbsp;<br></font></li><li><font style="font-size: 18px;">Perform above experiments with prepared plasma circuit &nbsp;&nbsp;<br></font></li><li><font style="font-size: 18px;">Titrate both circuit and trigger to find optimal mixture &nbsp;&nbsp;<br></font></li><li><font style="font-size: 18px;">Standardize reactions &nbsp; &nbsp;<br></font></li><li><font style="font-size: 18px;">Test on paper matrix</font><br></li></ul><p></p></div></div></div><div class="sDivider col-md-4 index2" data-colsize="4" data-title="Column"><div class="simblaEL simblaGallery" data-drag="P137" id="P137" data-g-hover-color="" data-g-text-color="" data-g-style="FADE" data-g-spacing="NORMAL"><div class="row simblaEL rDivider" data-title="Row"><div class="sDivider elem col-md-12 col-sm-12" data-title="Column"></div></div></div><div class="simblaEL tcH" data-drag="P141" id="P141" style="position: relative;"><div class="textContainer H2" style="margin-left: 10px; cursor: text;"><h2>PHASE 3</h2></div></div><div class="simblaEL tc" data-drag="P143" id="P143" style="position: relative;"><div class="textContainer" style="cursor: text; margin-left: 10px; line-height: 24px;"><p style="line-height: 24px;"></p><ul><li><font style="font-size: 18px;">Test efficacy of freeze drying circuit on paper for distribution and storage purposes. &nbsp;</font> &nbsp;<br></li></ul><p></p><p style="line-height: 24px;"><br></p></div></div></div></div></div></div></div></div><a href="https://www.simbla.com" target="_blank" id="linkToSimbla" class="en"><span class="main">SIMBLA</span><span class="s">Website Builder</span></a><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/jquery.bootpag.min.js"></script><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/bootstrap3.min.js"></script><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/Chart.min.js"></script><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/numeral.min.js"></script><script src="/static/js/simbla.js?v=2.0.4"></script><script src="https://www.google.com/recaptcha/api.js?onload=onloadRecaptcha&render=explicit" async defer></script></body>
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<h1>Design</h1>
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
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This page is different to the "Applied Design Award" page. Please see the <a href="https://2017.igem.org/Team:Cadets2Vets/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
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<h5>What should this page contain?</h5>
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<li>Explanation of the engineering principles your team used in your design</li>
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<h5>Inspiration</h5>
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<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
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<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
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<h1 id="vbid-ff3ca-dnp8mtfr" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Our circuit was designed to utilize specific parts so that when the E-coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light. <br></h1>
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<p>THE FOUNDATION OF OUR CIRCUIT</p>
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<ul style="box-sizing: border-box; line-height: 1.5em; list-style-type: square; margin: 0.3em 0px 0px 1.6em; padding-top: 0px; padding-right: 20px; padding-bottom: 0px; list-style-image: url(&quot;https://2017.igem.org/wiki/skins/Igem/resources/bullet.gif?2015-09-09T00:46:40Z&quot;); font-size: 13px; font-family: Tahoma, Geneva, sans-serif; color: rgb(0, 0, 0); text-align: start;"><li style="box-sizing: border-box; margin-bottom: 0.1em;"><span style="color:#F0FFFF;"><font style="box-sizing: border-box; font-size: 18px;">Test the efficacy of the plasma circuit in S.I.T.U.  </font></span></li><li style="box-sizing: border-box; margin-bottom: 0.1em;"><span style="color:#F0FFFF;"><font style="box-sizing: border-box; font-size: 18px;">Perform above experiments with prepared plasma circuit  </font></span></li><li style="box-sizing: border-box; margin-bottom: 0.1em;"><span style="color:#F0FFFF;"><font style="box-sizing: border-box; font-size: 18px;">Titrate both circuit and trigger to find optimal mixture  </font></span></li><li style="box-sizing: border-box; margin-bottom: 0.1em;"><span style="color:#F0FFFF;"><font style="box-sizing: border-box; font-size: 18px;">Standardize reactions    </font></span></li><li style="box-sizing: border-box; margin-bottom: 0.1em;"><span style="color:#F0FFFF;"><font style="box-sizing: border-box; font-size: 18px;">Test on paper matrix</font></span></li></ul>
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<h3 id="vbid-ff3ca-eap7ixkv" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">pHASE 1</h3>
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<ul style="box-sizing: border-box; line-height: 1.5em; list-style-type: square; margin: 0.3em 0px 0px 1.6em; padding-top: 0px; padding-right: 20px; padding-bottom: 0px; list-style-image: url(&quot;https://2017.igem.org/wiki/skins/Igem/resources/bullet.gif?2015-09-09T00:46:40Z&quot;); font-size: 13px; font-family: Tahoma, Geneva, sans-serif; color: rgb(0, 0, 0); text-align: start;"><li style="box-sizing: border-box; margin-bottom: 0.1em;"><span style="color:#F0FFF0;"><font style="box-sizing: border-box; font-size: 18px;">Test efficacy of the circuit in vivo </font></span></li><li style="box-sizing: border-box; margin-bottom: 0.1em;"><span style="color:#F0FFF0;"><font style="box-sizing: border-box; font-size: 18px;">Grow transformed e.coli with plasma circuit in Arsenic laced media  </font></span></li><li style="box-sizing: border-box; margin-bottom: 0.1em;"><span style="color:#F0FFF0;"><font style="box-sizing: border-box; font-size: 18px;">Serial dilution of arsenic and arsenic to find the lowest activation amount of the circuit in vivo</font></span></li></ul>
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<h3 id="vbid-ff3ca-85f0tohh" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">pHASE 3</h3>
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<ul style="box-sizing: border-box; line-height: 1.5em; list-style-type: square; margin: 0.3em 0px 0px 1.6em; padding-top: 0px; padding-right: 20px; padding-bottom: 0px; list-style-image: url(&quot;https://2017.igem.org/wiki/skins/Igem/resources/bullet.gif?2015-09-09T00:46:40Z&quot;); font-size: 13px; font-family: Tahoma, Geneva, sans-serif; color: rgb(0, 0, 0); text-align: start;"><li style="box-sizing: border-box; margin-bottom: 0.1em;"><span style="color:#F0FFF0;"><font style="box-sizing: border-box; font-size: 18px;">Test efficacy of freeze drying circuit on paper for distribution and storage purposes.  </font>  </span></li></ul>
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Revision as of 22:59, 19 June 2017

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Cadets2Vets

Design - DESIGN

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DESIGN



Our circuit was designed to utilize specific parts so that when the E-coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.





THE FOUNDATION OF OUR CIRCUIT



pHASE 2



  • Test the efficacy of the plasma circuit in S.I.T.U.
  • Perform above experiments with prepared plasma circuit
  • Titrate both circuit and trigger to find optimal mixture
  • Standardize reactions
  • Test on paper matrix


pHASE 1



  • Test efficacy of the circuit in vivo
  • Grow transformed e.coli with plasma circuit in Arsenic laced media
  • Serial dilution of arsenic and arsenic to find the lowest activation amount of the circuit in vivo


pHASE 3



  • Test efficacy of freeze drying circuit on paper for distribution and storage purposes.

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2304 Jefferson Ave, Tacoma, WA 98402