Difference between revisions of "Team:Cadets2Vets/Safety"

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<div class='strechH ' style='position:absolute;left:-182px;top:630px;z-index:10001;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;width:1601px;height:834px;overflow:hidden;background-color:rgb(0, 0, 0);background-position-x:undefined;background-position-y:undefined;background-repeat:undefined;background-attachment:undefined;border-width:0px;border-style:solid;border-color:transparent;border-color:rgb(0, 0, 0);-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:1526px;height:834px;'>
 
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<div class='ParagraphBox '  style='position:absolute;left:-27px;top:665px;z-index:10005;padding-left:14px;padding-right:15px;padding-top:5px;padding-bottom:5px;width:1378px;height:518px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:rgb(0, 0, 0);-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:1315px;height:518px;overflow:hidden;'><div class='innerText'><p style="margin-top: 0px; margin-left: 0px;"><span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular; font-size: 28px;'>Laboratory Practices </span></p><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;"><span style="font-size: 24px;"><span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Individuals must wash their hands: (a) after working with potentially hazardous materials, recombinant materials and animals; (b) after removing gloves; and (c) before leaving the laboratory. </span></span></p><p style="margin-top: 0px; margin-left: 0px;"><span style="font-size: 24px;"><span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Eating, drinking, smoking, handling contact lenses and applying cosmetics are not permitted in laboratory areas. </span></span></p><p style="margin-top: 0px; margin-left: 0px;"><span style="font-size: 24px;"><span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Mouth pipetting is prohibited; mechanical pipetting devices must be used. </span></span></p><p style="margin-top: 0px; margin-left: 0px;"><span style="font-size: 24px;"><span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• All procedures should be performed in such a way that minimizes splashes and/or aerosols. </span></span></p><p style="margin-top: 0px; margin-left: 0px;"><span style="font-size: 24px;"><span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Work can be performed on an open lab bench or table . </span></span></p><p style="margin-top: 0px; margin-left: 0px;"><span style="font-size: 24px;"><span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Decontaminate all work surfaces after completion of work and after any spill or splash of potentially infectious or viable recombinant material with appropriate disinfectant. Suitable disinfectants include: 10% bleach solution, 70% ethanol solution or 2% wescodyne solution. </span></span></p><p style="margin-top: 0px; margin-left: 0px;"><span style="font-size: 24px;"><span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Decontaminate all cultures, stocks, and other potentially infectious or recombinant materials before disposal using an effective method.</span></span></p></div>
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Revision as of 23:17, 19 June 2017

Cadets2Vets

Home

 

Safety

 

We designed our project specifically because of the safety hazard presented by arsenic pollution, and Tacoma's reputation of being a city with a relatively high level of Arsenic contamination.

 

 

 

 

 

 

 

 

 Lab Safety

Lab Safety Biosafety Level 1 (BSL-1)

Safety Guidelines If you work in a lab that is designated a BSL-1, the microbes there are not known to consistently cause disease in healthy adults and present minimal potential hazard to laboratory staff and the environment. An example of a microbe that is typically worked with at a BSL-1 is a nonpathogenic strain of E. coli

 

 

Laboratory Practices

 

 

• Individuals must wash their hands: (a) after working with potentially hazardous materials, recombinant materials and animals; (b) after removing gloves; and (c) before leaving the laboratory.

• Eating, drinking, smoking, handling contact lenses and applying cosmetics are not permitted in laboratory areas.

• Mouth pipetting is prohibited; mechanical pipetting devices must be used.

• All procedures should be performed in such a way that minimizes splashes and/or aerosols.

• Work can be performed on an open lab bench or table .

• Decontaminate all work surfaces after completion of work and after any spill or splash of potentially infectious or viable recombinant material with appropriate disinfectant. Suitable disinfectants include: 10% bleach solution, 70% ethanol solution or 2% wescodyne solution.

• Decontaminate all cultures, stocks, and other potentially infectious or recombinant materials before disposal using an effective method.

Safety

Please visit the main Safety page to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.

On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)

Safe Project Design

Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:

  • Choosing a non-pathogenic chassis
  • Choosing parts that will not harm humans / animals / plants
  • Substituting safer materials for dangerous materials in a proof-of-concept experiment
  • Including an "induced lethality" or "kill-switch" device
Safe Lab Work

What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!

Safe Shipment

Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?