Difference between revisions of "Team:IIT Delhi"

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    <h2 class="toppad">Abstract</h2>
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              <blockquote><p align = "left"  >Lack of digital responses in Synthetic Biology have inhibited the diverse potential that accompanies the digitization of biological circuits. This year we aim to develop synthetic modules for signal processing in biological systems, in the form of elements of specialized logic gates based on transcriptional regulation. We move from developing near digital logic gates with sharp responses, to more specialized collapsible and reconfigurable circuits which can perform various operations like developing square pulses. Further, to realize this aim of making a square wave generator, we engineered a five node repression based ring network to give digital oscillations. Quantitative computational modelling would be used to tailor the cellular environment and observe period, steepness, noise and amplitude variations. Our project poses to be an integral element in genetic networks intended to solve scientific challenges for years to come, ranging from making light sensitive frequency modulators and bacterial memory storage systems.</p>
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−
input[type="checkbox"]:checked + label:before,
 
−
input[type="radio"]:checked + label:before {
 
−
background: #3ba666;
 
−
border-color: #3ba666;
 
−
color: #ffffff;
 
−
content: '\f00c';
 
−
}
 
−
 
−
input[type="checkbox"]:focus + label:before,
 
−
input[type="radio"]:focus + label:before {
 
−
border-color: #4dac71;
 
−
box-shadow: 0 0 0 1px #4dac71;
 
−
}
 
−
 
−
input[type="checkbox"] + label:before {
 
−
border-radius: 4px;
 
−
}
 
−
 
−
input[type="radio"] + label:before {
 
−
border-radius: 100%;
 
−
}
 
−
 
−
::-webkit-input-placeholder {
 
−
color: #888 !important;
 
−
opacity: 1.0;
 
−
}
 
−
 
−
:-moz-placeholder {
 
−
color: #888 !important;
 
−
opacity: 1.0;
 
−
}
 
−
 
−
::-moz-placeholder {
 
−
color: #888 !important;
 
−
opacity: 1.0;
 
−
}
 
−
 
−
:-ms-input-placeholder {
 
−
color: #888 !important;
 
−
opacity: 1.0;
 
−
}
 
−
 
−
.formerize-placeholder {
 
−
color: #888 !important;
 
−
opacity: 1.0;
 
−
}
 
−
 
−
/* Box */
 
−
 
−
.box {
 
−
border-radius: 4px;
 
−
border: solid 1px rgba(144, 144, 144, 0.25);
 
−
margin-bottom: 2em;
 
−
padding: 1.5em;
 
−
}
 
−
 
−
.box > :last-child,
 
−
.box > :last-child > :last-child,
 
−
.box > :last-child > :last-child > :last-child {
 
−
margin-bottom: 0;
 
−
}
 
−
 
−
.box.alt {
 
−
border: 0;
 
−
border-radius: 0;
 
−
padding: 0;
 
−
}
 
−
 
−
/* Icon */
 
−
 
−
.icon {
 
−
text-decoration: none;
 
−
border-bottom: none;
 
−
position: relative;
 
−
}
 
−
 
−
.icon:before {
 
−
-moz-osx-font-smoothing: grayscale;
 
−
-webkit-font-smoothing: antialiased;
 
−
font-family: FontAwesome;
 
−
font-style: normal;
 
−
font-weight: normal;
 
−
text-transform: none !important;
 
−
}
 
−
 
−
.icon > .label {
 
−
display: none;
 
−
}
 
−
 
−
.icon.rounded {
 
−
border-radius: 100%;
 
−
border: 1px solid #4dac71;
 
−
display: inline-block;
 
−
height: 2em;
 
−
line-height: 2em;
 
−
text-align: center;
 
−
width: 2em;
 
−
}
 
−
 
−
.icon.rounded.big {
 
−
font-size: 3.5em;
 
−
}
 
−
 
−
/* Image */
 
−
 
−
.image {
 
−
border-radius: 4px;
 
−
border: 0;
 
−
display: inline-block;
 
−
position: relative;
 
−
}
 
−
 
−
.image img {
 
−
border-radius: 4px;
 
−
display: block;
 
−
}
 
−
 
−
.image.left {
 
−
float: left;
 
−
padding: 0 1.5em 1em 0;
 
−
top: 0.25em;
 
−
}
 
−
 
−
.image.right {
 
−
float: right;
 
−
padding: 0 0 1em 1.5em;
 
−
top: 0.25em;
 
−
}
 
−
 
−
.image.left, .image.right {
 
−
max-width: 40%;
 
−
}
 
−
 
−
.image.left img, .image.right img {
 
−
width: 100%;
 
−
}
 
−
 
−
.image.fit {
 
−
display: block;
 
−
margin: 0 0 2em 0;
 
−
width: 100%;
 
−
}
 
−
 
−
.image.fit img {
 
−
width: 100%;
 
−
}
 
−
 
−
/* List */
 
−
 
−
ol {
 
−
list-style: decimal;
 
−
margin: 0 0 2em 0;
 
−
padding-left: 1.25em;
 
−
}
 
−
 
−
ol li {
 
−
padding-left: 0.25em;
 
−
}
 
−
 
−
ul {
 
−
list-style: disc;
 
−
margin: 0 0 2em 0;
 
−
padding-left: 1em;
 
−
}
 
−
 
−
ul li {
 
−
padding-left: 0.5em;
 
−
}
 
−
 
−
ul.alt {
 
−
list-style: none;
 
−
padding-left: 0;
 
−
}
 
−
 
−
ul.alt li {
 
−
border-top: solid 1px rgba(144, 144, 144, 0.25);
 
−
padding: 0.8em 0;
 
−
}
 
−
 
−
ul.alt li:first-child {
 
−
border-top: 0;
 
−
padding-top: 0;
 
−
}
 
−
 
−
ul.icons {
 
−
cursor: default;
 
−
list-style: none;
 
−
padding-left: 0;
 
−
}
 
−
 
−
ul.icons li {
 
−
display: inline-block;
 
−
padding: 0 1em 0 0;
 
−
}
 
−
 
−
ul.icons li:last-child {
 
−
padding-right: 0;
 
−
}
 
−
 
−
ul.actions {
 
−
cursor: default;
 
−
list-style: none;
 
−
padding-left: 0;
 
−
}
 
−
 
−
ul.actions li {
 
−
display: inline-block;
 
−
padding: 0 1em 0 0;
 
−
vertical-align: middle;
 
−
}
 
−
 
−
ul.actions li:last-child {
 
−
padding-right: 0;
 
−
}
 
−
 
−
ul.actions.small li {
 
−
padding: 0 0.5em 0 0;
 
−
}
 
−
 
−
ul.actions.vertical li {
 
−
display: block;
 
−
padding: 1em 0 0 0;
 
−
}
 
−
 
−
ul.actions.vertical li:first-child {
 
−
padding-top: 0;
 
−
}
 
−
 
−
ul.actions.vertical li > * {
 
−
margin-bottom: 0;
 
−
}
 
−
 
−
ul.actions.vertical.small li {
 
−
padding: 0.5em 0 0 0;
 
−
}
 
−
 
−
ul.actions.vertical.small li:first-child {
 
−
padding-top: 0;
 
−
}
 
−
 
−
ul.actions.fit {
 
−
display: table;
 
−
margin-left: -1em;
 
−
padding: 0;
 
−
table-layout: fixed;
 
−
width: calc(100% + 1em);
 
−
}
 
−
 
−
ul.actions.fit li {
 
−
display: table-cell;
 
−
padding: 0 0 0 1em;
 
−
}
 
−
 
−
ul.actions.fit li > * {
 
−
margin-bottom: 0;
 
−
}
 
−
 
−
ul.actions.fit.small {
 
−
margin-left: -0.5em;
 
−
width: calc(100% + 0.5em);
 
−
}
 
−
 
−
ul.actions.fit.small li {
 
−
padding: 0 0 0 0.5em;
 
−
}
 
−
 
−
ul.tabular {
 
−
list-style: outside none none;
 
−
padding: 0px;
 
−
}
 
−
 
−
ul.tabular li {
 
−
border-top: solid 1px rgba(144, 144, 144, 0.25);
 
−
line-height: 1.75em;
 
−
margin: 1.5em 0px 0px;
 
−
padding-left: 7em;
 
−
padding-top: 12px;
 
−
position: relative;
 
−
}
 
−
 
−
ul.tabular li:first-child {
 
−
border-top: 0;
 
−
margin-top: 0;
 
−
}
 
−
 
−
ul.tabular li h3 {
 
−
left: 0px;
 
−
position: absolute;
 
−
text-align: center;
 
−
top: 12px;
 
−
vertical-align: top;
 
−
width: 1em;
 
−
}
 
−
 
−
dl {
 
−
margin: 0 0 2em 0;
 
−
}
 
−
 
−
/* Table */
 
−
 
−
.table-wrapper {
 
−
-webkit-overflow-scrolling: touch;
 
−
overflow-x: auto;
 
−
}
 
−
 
−
table {
 
−
margin: 0 0 2em 0;
 
−
width: 100%;
 
−
}
 
−
 
−
table tbody tr {
 
−
border: solid 1px rgba(144, 144, 144, 0.25);
 
−
border-left: 0;
 
−
border-right: 0;
 
−
}
 
−
 
−
table tbody tr:nth-child(2n + 1) {
 
−
background-color: rgba(144, 144, 144, 0.075);
 
−
}
 
−
 
−
table td {
 
−
padding: 0.75em 0.75em;
 
−
}
 
−
 
−
table th {
 
−
color: #666;
 
−
font-size: 0.9em;
 
−
font-weight: 400;
 
−
padding: 0 0.75em 0.75em 0.75em;
 
−
text-align: left;
 
−
}
 
−
 
−
table thead {
 
−
border-bottom: solid 0px rgba(144, 144, 144, 0.25);
 
−
}
 
−
 
−
table tfoot {
 
−
border-top: solid 2px rgba(144, 144, 144, 0.25);
 
−
}
 
−
 
−
table.alt {
 
−
border-collapse: separate;
 
−
}
 
−
 
−
table.alt tbody tr td {
 
−
border: solid 1px rgba(144, 144, 144, 0.25);
 
−
border-left-width: 0;
 
−
border-top-width: 0;
 
−
}
 
−
 
−
table.alt tbody tr td:first-child {
 
−
border-left-width: 1px;
 
−
}
 
−
 
−
table.alt tbody tr:first-child td {
 
−
border-top-width: 1px;
 
−
}
 
−
 
−
table.alt thead {
 
−
border-bottom: 0;
 
−
}
 
−
 
−
table.alt tfoot {
 
−
border-top: 0;
 
−
}
 
−
 
−
/* Button */
 
−
 
−
input[type="submit"],
 
−
input[type="reset"],
 
−
input[type="button"],
 
−
.button {
 
−
-moz-appearance: none;
 
−
-webkit-appearance: none;
 
−
-o-appearance: none;
 
−
-ms-appearance: none;
 
−
appearance: none;
 
−
-moz-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
 
−
-webkit-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
 
−
-o-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
 
−
-ms-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
 
−
transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
 
−
background-color: #3ba666;
 
−
border-radius: 4px;
 
−
border: 0;
 
−
color: #ffffff !important;
 
−
cursor: pointer;
 
−
display: inline-block;
 
−
font-weight: 400;
 
−
height: 2.85em;
 
−
line-height: 2.8em;
 
−
padding: 0 2em;
 
−
text-align: center;
 
−
text-decoration: none;
 
−
white-space: nowrap;
 
−
}
 
−
 
−
input[type="submit"]:hover,
 
−
input[type="reset"]:hover,
 
−
input[type="button"]:hover,
 
−
.button:hover {
 
−
background-color: #42b972;
 
−
}
 
−
 
−
input[type="submit"]:active,
 
−
input[type="reset"]:active,
 
−
input[type="button"]:active,
 
−
.button:active {
 
−
background-color: #34935a;
 
−
}
 
−
 
−
input[type="submit"].icon,
 
−
input[type="reset"].icon,
 
−
input[type="button"].icon,
 
−
.button.icon {
 
−
padding-left: 1.35em;
 
−
}
 
−
 
−
input[type="submit"].icon:before,
 
−
input[type="reset"].icon:before,
 
−
input[type="button"].icon:before,
 
−
.button.icon:before {
 
−
margin-right: 0.5em;
 
−
}
 
−
 
−
input[type="submit"].fit,
 
−
input[type="reset"].fit,
 
−
input[type="button"].fit,
 
−
.button.fit {
 
−
display: block;
 
−
margin: 0 0 1em 0;
 
−
width: 100%;
 
−
}
 
−
 
−
input[type="submit"].small,
 
−
input[type="reset"].small,
 
−
input[type="button"].small,
 
−
.button.small {
 
−
font-size: 0.8em;
 
−
}
 
−
 
−
input[type="submit"].big,
 
−
input[type="reset"].big,
 
−
input[type="button"].big,
 
−
.button.big {
 
−
font-size: 1.35em;
 
−
}
 
−
 
−
input[type="submit"].alt,
 
−
input[type="reset"].alt,
 
−
input[type="button"].alt,
 
−
.button.alt {
 
−
background-color: transparent;
 
−
box-shadow: inset 0 0 0 2px rgba(144, 144, 144, 0.25);
 
−
color: #666 !important;
 
−
}
 
−
 
−
input[type="submit"].alt:hover,
 
−
input[type="reset"].alt:hover,
 
−
input[type="button"].alt:hover,
 
−
.button.alt:hover {
 
−
background-color: rgba(144, 144, 144, 0.075);
 
−
}
 
−
 
−
input[type="submit"].alt:active,
 
−
input[type="reset"].alt:active,
 
−
input[type="button"].alt:active,
 
−
.button.alt:active {
 
−
background-color: rgba(144, 144, 144, 0.2);
 
−
}
 
−
 
−
input[type="submit"].alt.icon:before,
 
−
input[type="reset"].alt.icon:before,
 
−
input[type="button"].alt.icon:before,
 
−
.button.alt.icon:before {
 
−
color: #888;
 
−
}
 
−
 
−
input[type="submit"].special,
 
−
input[type="reset"].special,
 
−
input[type="button"].special,
 
−
.button.special {
 
−
background-color: #4dac71;
 
−
color: #ffffff !important;
 
−
}
 
−
 
−
input[type="submit"].special:hover,
 
−
input[type="reset"].special:hover,
 
−
input[type="button"].special:hover,
 
−
.button.special:hover {
 
−
background-color: #5cb67e;
 
−
}
 
−
 
−
input[type="submit"].special:active,
 
−
input[type="reset"].special:active,
 
−
input[type="button"].special:active,
 
−
.button.special:active {
 
−
background-color: #459a65;
 
−
}
 
−
 
−
input[type="submit"].disabled, input[type="submit"]:disabled,
 
−
input[type="reset"].disabled,
 
−
input[type="reset"]:disabled,
 
−
input[type="button"].disabled,
 
−
input[type="button"]:disabled,
 
−
.button.disabled,
 
−
.button:disabled {
 
−
background-color: #444 !important;
 
−
box-shadow: inset 0 -0.15em 0 0 rgba(0, 0, 0, 0.15);
 
−
color: #fff !important;
 
−
cursor: default;
 
−
opacity: 0.25;
 
−
}
 
−
 
−
/* Header */
 
−
 
−
body.landing #header {
 
−
background-color: transparent;
 
−
left: 0;
 
−
position: absolute;
 
−
top: 0;
 
−
}
 
−
 
−
#header {
 
−
background-color: #202222;
 
−
color: #ffffff;
 
−
cursor: default;
 
−
height: 4.75em;
 
−
line-height: 4.75em;
 
−
width: 100%;
 
−
z-index: 10000;
 
−
}
 
−
 
−
#header h1 {
 
−
color: #ffffff;
 
−
height: inherit;
 
−
left: 2.5em;
 
−
line-height: inherit;
 
−
margin: 0;
 
−
padding: 0;
 
−
position: absolute;
 
−
top: 0;
 
−
}
 
−
 
−
#header h1 a {
 
−
font-size: 1.25em;
 
−
}
 
−
 
−
#header nav {
 
−
height: inherit;
 
−
line-height: inherit;
 
−
position: absolute;
 
−
right: 2.75em;
 
−
top: 0;
 
−
vertical-align: middle;
 
−
}
 
−
 
−
#header nav > ul {
 
−
list-style: none;
 
−
margin: 0;
 
−
padding-left: 0;
 
−
}
 
−
 
−
#header nav > ul > li {
 
−
border-radius: 4px;
 
−
display: inline-block;
 
−
margin-left: 1.5em;
 
−
padding-left: 0;
 
−
}
 
−
 
−
#header nav > ul > li a {
 
−
-moz-transition: color 0.2s ease-in-out;
 
−
-webkit-transition: color 0.2s ease-in-out;
 
−
-o-transition: color 0.2s ease-in-out;
 
−
-ms-transition: color 0.2s ease-in-out;
 
−
transition: color 0.2s ease-in-out;
 
−
color: #cee8d8;
 
−
display: inline-block;
 
−
text-decoration: none;
 
−
}
 
−
 
−
#header nav > ul > li a:hover {
 
−
color: #ffffff;
 
−
}
 
−
 
−
#header nav > ul > li:first-child {
 
−
margin-left: 0;
 
−
}
 
−
 
−
#header nav > ul > li .button {
 
−
height: 2.25em;
 
−
line-height: 2.25em;
 
−
margin-bottom: 0;
 
−
padding: 0 1em;
 
−
position: relative;
 
−
top: -0.075em;
 
−
vertical-align: middle;
 
−
}
 
−
 
−
#header .container {
 
−
position: relative;
 
−
}
 
−
 
−
#header .container h1 {
 
−
left: 0;
 
−
}
 
−
 
−
#header .container nav {
 
−
right: 0;
 
−
}
 
−
 
−
/* Banner */
 
−
 
−
#banner {
 
−
background: url("../images/banner.jpg") ;
 
−
 
−
background-position: center;
 
−
background-repeat: no-repeat;
 
−
background-size: cover;
 
−
color: #ffffff;
 
−
padding: 41em 0em 1em;
 
−
text-align: center;
 
−
 
−
}
 
−
 
−
#banner h2 {
 
−
color: #ffffff;
 
−
font-size: 3em;
 
−
line-height: 1em;
 
−
margin: 0 0 0.5em 0;
 
−
padding: 0;
 
−
}
 
−
 
−
#banner p {
 
−
font-size: 1.5em;
 
−
margin: 1.5em 0 0 0;
 
−
}
 
−
 
−
#banner .actions {
 
−
margin: 3em 0 0 0;
 
−
}
 
−
 
−
#banner .button {
 
−
background-color: transparent;
 
−
border: 1px solid #ffffff;
 
−
font-weight: 300;
 
−
}
 
−
 
−
#banner .button:hover {
 
−
background-color: rgba(255, 255, 255, 0.2);
 
−
}
 
−
 
−
/* Wrapper */
 
−
 
−
.wrapper {
 
−
padding: 3em 0em 4em;
 
−
}
 
−
 
−
.wrapper.style1 {
 
−
background-color: #3ba666;
 
−
-moz-background-image: linear-gradient(60deg, #4dac71 50%, #3ba666 50%);
 
−
-webkit-background-image: linear-gradient(60deg, #4dac71 50%, #3ba666 50%);
 
−
-o-background-image: linear-gradient(60deg, #4dac71 50%, #3ba666 50%);
 
−
-ms-background-image: linear-gradient(60deg, #4dac71 50%, #3ba666 50%);
 
−
background-image: linear-gradient(60deg, #4dac71 50%, #3ba666 50%);
 
−
color: #ffffff;
 
−
margin-top: 0em;
 
−
}
 
−
 
−
.wrapper.style1 header {
 
−
margin-bottom: 4em;
 
−
}
 
−
 
−
.wrapper.style1 h2 {
 
−
color: #ffffff;
 
−
}
 
−
 
−
.wrapper.style1 .icon.big {
 
−
border-color: #ffffff;
 
−
margin-bottom: 0.5em;
 
−
}
 
−
 
−
.wrapper.style2 .title, .wrapper.style2 header {
 
−
border-bottom: medium double rgba(144, 144, 144, 0.25);
 
−
padding-bottom: 1em;
 
−
}
 
−
 
−
.wrapper.style2 header {
 
−
margin-bottom: 4em;
 
−
}
 
−
 
−
.wrapper.style2 .feature {
 
−
margin: 0 0 2em 0;
 
−
}
 
−
 
−
/* Footer */
 
−
 
−
#footer {
 
−
background: #202222;
 
−
color: #ddd;
 
−
padding: 1em 0em 1em;
 
−
}
 
−
 
−
#footer h2, #footer h3, #footer h4 {
 
−
color: #ffffff;
 
−
}
 
−
 
−
#footer a {
 
−
-moz-transition: all 0.2s ease-in-out;
 
−
-webkit-transition: all 0.2s ease-in-out;
 
−
-o-transition: all 0.2s ease-in-out;
 
−
-ms-transition: all 0.2s ease-in-out;
 
−
transition: all 0.2s ease-in-out;
 
−
color: #888;
 
−
}
 
−
 
−
#footer a:active, #footer a:hover {
 
−
color: #ffffff;
 
−
}
 
−
 
−
#footer .icons .rounded {
 
−
border-color: #888;
 
−
font-size: 1.5em;
 
−
}
 
−
 
−
#footer .icons .rounded:active, #footer .icons .rounded:hover {
 
−
border-color: #ffffff;
 
−
}
 
−
 
−
#footer .copyright {
 
−
font-size: 0.9em;
 
−
margin: 2em 0 0 0;
 
−
padding: 0;
 
−
text-align: center;
 
−
}
 
−
 
−
#footer .copyright li {
 
−
border-left: solid 1px rgba(144, 144, 144, 0.25);
 
−
display: inline-block;
 
−
list-style: none;
 
−
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<p ><b>E-mail:</b> iitd.igem@gmail.com<br>Undergraduate Laboratory<br>Department of Biotechnology and Biochemical Engineering, IIT Delhi
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Revision as of 20:38, 27 October 2017

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</style> iGEM IIT Delhi

iGEM IIT Delhi
Description Results Proof of Concept Basic Parts Composite Parts
Overview Logic Assessment Signalling Tools
Human Practices Integrated Practices Public Engagement Collaborations
Lab Records Protocols Recipes Medal Criteria Recipes
The Team Attributions

Abstract

Lack of digital responses in Synthetic Biology have inhibited the diverse potential that accompanies the digitization of biological circuits. This year we aim to develop synthetic modules for signal processing in biological systems, in the form of elements of specialized logic gates based on transcriptional regulation. We move from developing near digital logic gates with sharp responses, to more specialized collapsible and reconfigurable circuits which can perform various operations like developing square pulses. Further, to realize this aim of making a square wave generator, we engineered a five node repression based ring network to give digital oscillations. Quantitative computational modelling would be used to tailor the cellular environment and observe period, steepness, noise and amplitude variations. Our project poses to be an integral element in genetic networks intended to solve scientific challenges for years to come, ranging from making light sensitive frequency modulators and bacterial memory storage systems.

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Contact Us Address

E-mail: iitd.igem@gmail.com
Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi