CharisKomos (Talk | contribs) |
CharisKomos (Talk | contribs) |
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#sub4{ color: orange; list-style-image:none; list-style-type: disc; } | #sub4{ color: orange; list-style-image:none; list-style-type: disc; } | ||
#sub4 span{ color: black; } | #sub4 span{ color: black; } | ||
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+ | #sub5{ color: brown; list-style-image:none; list-style-type: disc; } | ||
+ | #sub5 span{ color: black; } | ||
#main_list_1 > li{ background: url('https://static.igem.org/mediawiki/2017/3/3c/Greekom_bronze_medal_criteria.png') no-repeat left top; padding: 22px 0px 0px 70px; list-style: none; margin: 10px 0px 0px 0px; } | #main_list_1 > li{ background: url('https://static.igem.org/mediawiki/2017/3/3c/Greekom_bronze_medal_criteria.png') no-repeat left top; padding: 22px 0px 0px 70px; list-style: none; margin: 10px 0px 0px 0px; } | ||
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</li> | </li> | ||
<li style='padding-bottom: 40px'><span><b>Improve a previous part or project:</b> </span></li> | <li style='padding-bottom: 40px'><span><b>Improve a previous part or project:</b> </span></li> | ||
− | <li><span><b>Model our project:</b> The entirety of our classifier circuit design, ranging from miRNA selection to circuit topology, was based on insights we gained from our modeling. </span>< | + | <li><span><b>Model our project:</b> The entirety of our classifier circuit design, ranging from miRNA selection to circuit topology, was based on insights we gained from our modeling. </span> <ul id='sub5'> |
− | + | <li><span>Furthermore, we designed our experimental protocols for bacterial invasion and transfection using data obtained from our models. You can read all about <a href='https://2017.igem.org/Team:Greece/RNAi_Classifier_Design'>our models</a> and how we integrated them to our <a href='https://2017.igem.org/Team:Greece/ProjectDesign'>design</a>.</span></li> | |
+ | </ul> | ||
+ | </li> | ||
<li><span><b>Demonstrate your project works:</b> In our proof of concept experiment, we transformed Caco-2, the cell line that our logic circuit classifies, along with A549 and HEK-293 as controls with our RNAi-based classifier and demonstrated that our circuit can indeed perform identification and subsequent actuation of the pre-programmed response. In addition, we successfully utilized our engineered bacteria to achieve transfection of a plasmid coding for a fluorescent protein to our cell lines. You can read more about our results <a href='https://2017.igem.org/Team:Greece/Results'>here</a>.</span></li> | <li><span><b>Demonstrate your project works:</b> In our proof of concept experiment, we transformed Caco-2, the cell line that our logic circuit classifies, along with A549 and HEK-293 as controls with our RNAi-based classifier and demonstrated that our circuit can indeed perform identification and subsequent actuation of the pre-programmed response. In addition, we successfully utilized our engineered bacteria to achieve transfection of a plasmid coding for a fluorescent protein to our cell lines. You can read more about our results <a href='https://2017.igem.org/Team:Greece/Results'>here</a>.</span></li> | ||
</ul> | </ul> |
Revision as of 22:37, 27 October 2017
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