Since there are many kinds of E.coli in our system, it is difficult to understand the behavior of the system directly without model. Also, with an appropriate model, we can combine it with our experiment results to help us design our gene circuit.
    First, to adapt to our experiment design, we construct a complete fluid model which describes the growth of E.coli inside the fluid medium using Ordinary Differential Equation (ODE). This complete fluid model can help us describe the change of all six character’s amount. Furthermore, the fluid E.coli War is based on this model.
    As is stated in the orthogonality test in experiment part, we failed to find the orthometric gene circuit for two warriors. How can we solve this problem? Our model can tell us answer!
    However, complete fluid model is too complicated to instruct our experiment design. Therefore, we extracted parts of the model to help us design a good warrior I which just respond to the AHL secreted by warrior II but not itself. This can be seen on the page of Improved orthometric gene circuit.What’s more, the simplified model tells us how to regulate the killing ability of warrior experimentally.Detailed information is displayed on the page of Regulation of killing ability.
    Apart from things mentioned above, spatial factors also have a great impact on the results of population competition and cooperation. To understand spatial effects better, we developed our discrete probabilistic solid model, and this model is used as the basis of our solid E.coli War....