Difference between revisions of "Team:WHU-China/Composite Part"

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The biosensor made by Peking-China: http://parts.igem.org/Part:BBa_J61051
 
The biosensor made by Peking-China: http://parts.igem.org/Part:BBa_J61051
 
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<p align="center"> <img src="https://static.igem.org/mediawiki/2017/2/2b/WHU-China-Composite.png" width="800" height="200" alt=""/>
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<p align="center"> <img src="https://static.igem.org/mediawiki/2017/2/2b/WHU-China-Composite.png" width="800" height="150" alt=""/>
  
 
<h2>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Brick7 Combination of promoters in brick4 (BBa_K2462004) with RBS（BBa_B0030） (BBa_K2462006)</h2>
 
<h2>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Brick7 Combination of promoters in brick4 (BBa_K2462004) with RBS（BBa_B0030） (BBa_K2462006)</h2>

Revision as of 06:54, 28 October 2017

Composite Parts

         Brick2 RdhANP combined with a biosensor (BBa_K2462007)

         As we designed our project, we have wondered if there exists any regulation mechanism to make the expression of RdhANP more precisely. We searched the information and find a biosensor created by team Peking-China in 2013. This biosensor can function when it runs into substances analogous to salicylic acid. And some of the substances they tested can serve as the substrates of RdhANP , like 2,4,6-trichloro-phenol. We then linked this sensor(BBa_J61051) with RdhANP and the enzyme is supposed to express when it's substrates are available. The biosensor made by Peking-China: http://parts.igem.org/Part:BBa_J61051

         Brick7 Combination of promoters in brick4 (BBa_K2462004) with RBS（BBa_B0030） (BBa_K2462006)

         We improve a previous part(BBa_B0030) by combining it with the promoter used in Bacillus megaterium. As the strength of RBS(BBa_B0030) has already been tested and the promoters of these two composite bricks are suitable for Bacillus megaterium, it’s quite convenient for other teams to use.