Difference between revisions of "Team:KUAS Korea/Design"
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<h4> Detection System 1: hssR/S Mechanism </h4> | <h4> Detection System 1: hssR/S Mechanism </h4> | ||
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<img src="https://static.igem.org/mediawiki/2017/a/a9/T--KUAS_Korea--HrtR.png"> | <img src="https://static.igem.org/mediawiki/2017/a/a9/T--KUAS_Korea--HrtR.png"> | ||
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<p><font size=4>Also, the mechanism of heme detection using hrtR protein is as follows.</font></p> | <p><font size=4>Also, the mechanism of heme detection using hrtR protein is as follows.</font></p> | ||
<p><font size=4> ①If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> | <p><font size=4> ①If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> | ||
Revision as of 07:29, 28 October 2017
Design
POO-robiotics
Detection System 1: hssR/S Mechanism
The hssR and hssS mechanism of our project is as follows.
① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.
③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.
④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.
Detection System 2: hrtR Mechanism
Also, the mechanism of heme detection using hrtR protein is as follows.
①If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.
③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.
