Difference between revisions of "Team:KUAS Korea/Safety"

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<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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    <h3>Safety</h3>
 
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<h5>Safe Project Design</h5>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<p><font size=4> We have thoughtfully considered the safety issues during our whole lab project. Details are as follows. </font> </p><br><br>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<p><font size=4> 1. Members of two-component regulatory system <strong>HssS/HssR</strong> are highly unlikely to be dangerous. They function as heme response acceptor and activate the expression of <strong>hrtAB</strong>, an efflux pump that indicates hemin and detoxifies heme stress. They are expressed in a strain of <em>Bacillus cereus</em>, which is classified as <strong>risk group 2</strong>.  </font></p><br>
  
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<p><font size=4> 2. <strong>HrtR protein</strong> is unlikely to be hazardous. It is an intracellular heme-binding protein which controls heme efflux by the conserved <strong>HrtAB transporter</strong>. They are expressed in a strain of <em>Lactococcus lactis</em>, which is classified as <strong>risk group 1</strong>. </font></p><br>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<p><font size=4> 3. <em>Bacillus cereus</em> KCTC3624 is a dangerous pathogenic species that produce toxin. It could cause food poisoning, vomiting, and diarrhea. This risky pathogen is classified as <strong>risk group 2</strong>. </font></p><br>
  
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<p><font size=4> 4. <em>Lactobacillus plantarum</em> L67 is a nonpathogenic species which is classified as <strong>risk group 1</strong>. This bacteria is responsible for the fermentative conversion of sugars in raw materials into lactic acid. It is commonly found in dairy, meat, vegetable fermentations and human gastrointestinal tract. </font></p><br><br>
  
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<p><font size=4> All of the chemicals we use are not dangerous. However, we deal with dangerous pathogenic organism <em>B.cereus</em> as a chassis. In order to protect ourselves, we always use gloves for protection and wear lab coats. We also always sterilize our experiment tools and biological materials to prevent the dispersion of hazardous materials. </font>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 09:03, 28 October 2017

Safety

We have thoughtfully considered the safety issues during our whole lab project. Details are as follows.



1. Members of two-component regulatory system HssS/HssR are highly unlikely to be dangerous. They function as heme response acceptor and activate the expression of hrtAB, an efflux pump that indicates hemin and detoxifies heme stress. They are expressed in a strain of Bacillus cereus, which is classified as risk group 2.


2. HrtR protein is unlikely to be hazardous. It is an intracellular heme-binding protein which controls heme efflux by the conserved HrtAB transporter. They are expressed in a strain of Lactococcus lactis, which is classified as risk group 1.


3. Bacillus cereus KCTC3624 is a dangerous pathogenic species that produce toxin. It could cause food poisoning, vomiting, and diarrhea. This risky pathogen is classified as risk group 2.


4. Lactobacillus plantarum L67 is a nonpathogenic species which is classified as risk group 1. This bacteria is responsible for the fermentative conversion of sugars in raw materials into lactic acid. It is commonly found in dairy, meat, vegetable fermentations and human gastrointestinal tract.



All of the chemicals we use are not dangerous. However, we deal with dangerous pathogenic organism B.cereus as a chassis. In order to protect ourselves, we always use gloves for protection and wear lab coats. We also always sterilize our experiment tools and biological materials to prevent the dispersion of hazardous materials.