Difference between revisions of "Team:KUAS Korea/Contribution"

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    <script type="text/javascript" src="//2016.igem.org/Team:Korea_U_Seoul/Assets/d3js?action=raw&ctype=text/javascript"></script>
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<div class="container-fluid page-heading" style="background-image: url(https://static.igem.org/mediawiki/2017/7/77/T--KUAS_Korea--Contribution.jpeg)">
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        <h3>Contribution</h3>
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<h4 id="exp"> Team KUAS_Korea 2017: characterization </h4>
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<p><font size=4>We tested the functionality of K1033222 as a constitutive promoter and expression of amilCP chromoprotein in the <i>Escherichia coli</i> DH5alpha.
  
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DH5alpha containing the control(pSB1C3) and BBa_K1033282, respectively, were cultured in LB medium containing chloramphenicol.
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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Absorbances were measured every 30 minutes using BioRad SmartSpecPlus at 588 nm and 800 nm.</font></p>
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<h1>Contribution</h1>
 
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<h3>Bronze Medal Criterion #4</h3>
 
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
 
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<br><br>
 
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<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
 
  
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                        <img src="https://static.igem.org/mediawiki/2017/d/d1/T--KUAS_Korea--Contribution1.jpeg">
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                    </div>
  
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                        <img src="https://static.igem.org/mediawiki/2017/b/ba/T--KUAS_Korea--Contribution2.png">
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                        <img src="https://static.igem.org/mediawiki/2017/3/30/T--KUAS_Korea--Contribution3.png">
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                    </div>
  
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                        <img src="https://static.igem.org/mediawiki/2017/1/18/T--KUAS_Korea--Contribution4.png">
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                    </div>
  
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<p><font size=4>The first photo is a transformation of BBa_K1033282 to DH5alpha. This was then grown in LB medium supplemented with chloramphenicol at 37 degrees Celsius. The first graph shows changes in OD588 over time, the second shows changes in OD588 / OD800 ratio with time, and the third shows OD588 according to OD800.
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{{:Team:KUAS_Korea/Templates/Sponsors}}

Latest revision as of 10:46, 28 October 2017

Contribution



Team KUAS_Korea 2017: characterization


We tested the functionality of K1033222 as a constitutive promoter and expression of amilCP chromoprotein in the Escherichia coli DH5alpha. DH5alpha containing the control(pSB1C3) and BBa_K1033282, respectively, were cultured in LB medium containing chloramphenicol. Absorbances were measured every 30 minutes using BioRad SmartSpecPlus at 588 nm and 800 nm.


The first photo is a transformation of BBa_K1033282 to DH5alpha. This was then grown in LB medium supplemented with chloramphenicol at 37 degrees Celsius. The first graph shows changes in OD588 over time, the second shows changes in OD588 / OD800 ratio with time, and the third shows OD588 according to OD800.