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<li> <a href="http://parts.igem.org/Part:BBa_K431009">BBa_K431009</a>,the pGAP promoter, in the chassis <i>Pichia pastoris</i>, thanks to RTq-PCR experiments. </li> | <li> <a href="http://parts.igem.org/Part:BBa_K431009">BBa_K431009</a>,the pGAP promoter, in the chassis <i>Pichia pastoris</i>, thanks to RTq-PCR experiments. </li> | ||
<li> <a href="http://parts.igem.org/Part:BBa_K1800001">BBa_K1800001</a>, an Alpha-Factor Secretion Signal, in the chassis <i>Pichia pastoris</i> thanks to toxicity assays. </li> | <li> <a href="http://parts.igem.org/Part:BBa_K1800001">BBa_K1800001</a>, an Alpha-Factor Secretion Signal, in the chassis <i>Pichia pastoris</i> thanks to toxicity assays. </li> | ||
− | <li> <a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1072010">BBa_K1072010</a>, an Odr-10(diacetyl) receptor in the chassis <i>P. pastoris< | + | <li> <a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1072010">BBa_K1072010</a>, an Odr-10(diacetyl) receptor in the chassis <i>P. pastoris</i>, thanks to fluorescence essays in a microplate reader. </li> |
<li> <a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1072023">BBa_K1072023</a>, the pFUS1 Promoter, in the chassis <i>P. pastoris</i>, thanks to fluorescence essays in a microplate reader. </li> | <li> <a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1072023">BBa_K1072023</a>, the pFUS1 Promoter, in the chassis <i>P. pastoris</i>, thanks to fluorescence essays in a microplate reader. </li> | ||
</ul> | </ul> | ||
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<img src="https://static.igem.org/mediawiki/2017/7/73/T--INSA-UPS_France--Achievements_Silver.png" style="width:200px; position:absolute; top:-100px; left:-70px;" alt=""> | <img src="https://static.igem.org/mediawiki/2017/7/73/T--INSA-UPS_France--Achievements_Silver.png" style="width:200px; position:absolute; top:-100px; left:-70px;" alt=""> | ||
<p> | <p> | ||
− | <b>#1: </b>Out of | + | <b>#1: </b>Out of 19 Parts, we obtained 6 new BioBricks compatible with the Registry. We successfully validated 3 of them: |
</p> | </p> | ||
+ | <ul> | ||
+ | <li> <a href="http://parts.igem.org/Part:BBa_K2278001">BBa_K2278001</a>, <i>V. harveyi</i> C8-CAI-1 (quorum sensing inducer) generator, with bioluminescence essays.</li> | ||
+ | <li> <a href="http://parts.igem.org/Part:BBa_K2278021">BBa_K2278021</a>, coding for the D-NY15 Antimicrobial peptide with an Alpha-Factor Secretion Signal. Our favorite Part was validated thanks to sequencing, RTq-PCR experiments and toxicity assays on Petri dishes.</li> | ||
+ | <li> <a href="http://parts.igem.org/Part:BBa_K2278011">BBa_K2278011</a>, pTet driven Diacetyl generator, validated thanks to NMR analysis.</li> | ||
+ | </ul> | ||
<p> | <p> | ||
<b>#2: </b>We are proud of all of the <a href="https://2017.igem.org/Team:INSA-UPS_France/Collaborations">collaborations</a> we made with teams around the world: | <b>#2: </b>We are proud of all of the <a href="https://2017.igem.org/Team:INSA-UPS_France/Collaborations">collaborations</a> we made with teams around the world: |
Revision as of 16:08, 28 October 2017
#1: We successfully registered for the competition, had an awesome summer and proudly attended the Giant Jamboree!
#2: All the required competition deliverables were met in time for the due dates:
#3: We created an Attribution page entirely designed to thank all the people that helped us throughout our adventure.
#4: We successfully characterised 5 existing parts:
#1: Out of 19 Parts, we obtained 6 new BioBricks compatible with the Registry. We successfully validated 3 of them:
#2: We are proud of all of the collaborations we made with teams around the world:
#3: We carried out an ethical reflexion throughout all of our project, doing bibliographical research and meeting professionals and members of NGOs. Our major discussions and decisions are listed in our Human Practices Logbook.
#1: Our ethical reflexion went even further with the elaboration of 7 ethical matrices. Each matrix was a representation of our personal reflection on one key step of a water treatment product lifecycle. The outcomes of each matrix was carefully integrated in our project and could reshape part of it if needed.
#2: Modeling was a big part of our project, unavoidable considering the synthetic interactions of our three chassis. After building a standardized representation of all the genetic and molecular actors involved in communications, the challenge was to verify and simulate the functioning of our system through Ordinary Differential Equations. Then, sensitivity and robustness was analyzed thanks to Metabolic Control Analysis. Finally, a user-friendly interface was created for people non familiarized with ODEs.
Medal Criteria
Bronze medal
Silver medal
Gold medal