Difference between revisions of "Team:BOKU-Vienna/Notebook"

Line 190: Line 190:
 
<br>• PCR: 1, 2, 3, 4, 6, 15, 18, 20, 23, 24, 25, 26</br>
 
<br>• PCR: 1, 2, 3, 4, 6, 15, 18, 20, 23, 24, 25, 26</br>
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
−
<br><br>• Golden Gate Assembly: BB1_09, BB1_10, BB1_11, BB1_12, BB1_13, BB2_01(unsuccessful), BB2_02, BB2_03, BB2_04, BB2_05 (unsuccessful)</br>
+
<br><br>• Golden Gate Assembly: BB1_09, BB1_10, BB1_11, BB1_12, BB1_13, BB2_01 (unsuccessful), BB2_02, BB2_03, BB2_04, BB2_05 (unsuccessful)</br>
 
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• pSIM5 pre-cultures (DH5alpha + BL21(DE3))</br>
 
<br>• pSIM5 pre-cultures (DH5alpha + BL21(DE3))</br>
Line 202: Line 202:
 
<br>• PCR: 27, 28</br>
 
<br>• PCR: 27, 28</br>
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
−
<br><br>• Golden Gate Assembly: BB1_14, BB1_15, BB2_01 (unsuccessful, even with new BBa_23101 promoter), BB2_05(unsuccessful), BB2_06, BB2_07, BB2_08, BB2_09, BB2_10, BB2_12, BB2_14, BB2_15, BB2_16, CRISPR (unsuccessful)</br>
+
<br><br>• Golden Gate Assembly: BB1_14, BB1_15, BB2_01 (unsuccessful, even with new BBa_23101 promoter), BB2_05 (unsuccessful), BB2_06, BB2_07, BB2_08, BB2_09, BB2_10, BB2_12, BB2_14, BB2_15, BB2_16, CRISPR (unsuccessful)</br>
 
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• Sent for Sequencing: BB1_14, BB1_15, BB2_01, BB2_05, BB2_06, BB2_07, BB2_08, BB2_09, BB2_10, BB2_12</br>
 
<br>• Sent for Sequencing: BB1_14, BB1_15, BB2_01, BB2_05, BB2_06, BB2_07, BB2_08, BB2_09, BB2_10, BB2_12</br>

Revision as of 19:42, 28 October 2017

Notebook

V

Notebook.


All protocols we used for the methods can be found here: Protocol 

Week 1 (03/07-07/07)
   
Week 2 (10/07-14/07)
   
Week 3 (17/07-21/07)
   
Week 4 (24/07-28/07)
   
Week 5 (31/07-04/08)
   
Week 6 (07/08-11/08)
   
Week 7 (14/08-18/08)
   
Week 8 (21/08-25/08)
   
Week 9 (28/08-01/09)
   
Week 10 (04/09-08/09)
   
Week 11 (11/09-15/09)
   
Week 12 (18/09-22/09)
   
Week 13 (25/09-29/09)