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Revision as of 20:46, 28 October 2017
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iGEM's interlab study aims to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. This means teams have to use the same exact protocol around the world to produce common, comparable units for measuring GFP with different plate readers. For more information on the protocol, check out iGEM's official Interlab Study webpage. Below is the data that we collected for the Interlab Study:
LUDOX-HS40 | H2O | |
---|---|---|
Replicate 1 | 0.0932 | 0.0973 |
Replicate 2 | 0.0927 | 0.0777 |
Replicate 3 | 0.0961 | 0.0796 |
Replicate 4 | 0.0937 | 0.0803 |
Arith. Mean | 0.093925 | 0.083725 |
Corrected Abs600 | 0.0102 | |
Reference OD600 | 0.0425 | |
OD600/Abs600 | 4.166666667 |
A1 | A2 | A3 | A4 | A5 | A6 | A7 | A8 | A9 |
B1 | B2 | B3 | B4 | B5 | B6 | B7 | B8 | B9 |
C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | C9 |
D1 | D2 | D3 | D4 | D5 | D6 | D7 | D8 | D9 |
E1 | E2 | E3 | E4 | E5 | E6 | E7 | E8 | E9 |
F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 |
G1 | G2 | G3 | G4 | G5 | G6 | G7 | G8 | G9 |
H1 | H2 | H3 | H4 | H5 | H6 | H7 | H8 | H9 |
uM Fluorescein/a.u. | 50.00 | 25 | 12.5 | 6.25 | 3.125 | 1.5625 | 0.78125 | 0.390625 | 0.1953125 | 0.09765625 | 0.048828125 |
---|---|---|---|---|---|---|---|---|---|---|---|
Mean um Fluorescein/a.u. | 0.000787755 | 0.000550785 | 0.000413815 | 0.000344908 | 0.000324583 | 0.000321304 | 0.000307518 | 0.000288497 | 0.000282243 | 0.00028492 | 0.00027862 |
Mean of med-high levels: | 0.000391079 |
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.0935 | 0.0951 | 0.0942 | 0.0917 | 0.0918 | 0.0925 | 0.0919 | 0.0933 | 0.0902 |
C1, R2 | 0.0937 | 0.0937 | 0.0973 | 0.0941 | 0.0937 | 0.096 | 0.0937 | 0.0945 | 0.0886 |
C1, R3 | 0.0946 | 0.0939 | 0.1013 | 0.0957 | 0.0931 | 0.1026 | 0.1074 | 0.0945 | 0.0904 |
C1, R4 | 0.0982 | 0.0943 | 0.0996 | 0.0942 | 0.0926 | 0.0939 | 0.094 | 0.0953 | 0.0899 |
C2, R1 | 0.0932 | 0.0944 | 0.0956 | 0.0933 | 0.0927 | 0.0919 | 0.0934 | .0973 | 0.0894 |
C2, R2 | 0.0928 | 0.0949 | 0.0975 | 0.0967 | 0.0976 | 0.0949 | 0.0962 | 0.0957 | 0.0894 |
C2, R3 | 0.0925 | 0.094 | 0.0983 | 0.095 | 0.0939 | 0.0941 | 0.0946 | 0.0955 | 0.0916 |
C2, R4 | 0.0918 | 0.0965 | 0.0958 | 0.0942 | 0.0939 | 0.0923 | 0.093 | 0.0932 | 0.0887 |
Hour 2: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.1289 | 0.1165 | 0.0932 | 0.1002 | 0.1056 | 0.0952 | 0.1129 | 0.1145 | 0.0892 |
C1, R2 | 0.1294 | 0.1158 | 0.0975 | 0.1031 | 0.1087 | 0.0973 | 0.1136 | 0.117 | 0.0922 |
C1, R3 | 0.1305 | 0.1156 | 0.0962 | 0.1013 | 0.108 | 0.0982 | 0.1157 | 0.1174 | 0.0916 |
C1, R4 | 0.1275 | 0.1153 | 0.096 | 0.1019 | 0.11 | 0.0964 | 0.1115 | 0.1189 | 0.0845 |
C2, R1 | 0.1273 | 0.1221 | 0.0957 | 0.1027 | 0.1165 | 0.0956 | 0.1147 | 0.1118 | 0.0844 |
C2, R2 | 0.129 | 0.1192 | 0.0958 | 0.1033 | 0.1198 | 0.0935 | 0.1162 | 0.114 | 0.0922 |
C2, R3 | 0.1246 | 0.1221 | 0.0961 | 0.1021 | 0.1178 | 0.096 | 0.1156 | 0.1114 | 0.0921 |
C2, R4 | 0.1258 | 0.1227 | 0.0969 | 0.0995 | 0.121 | 0.096 | 0.1139 | 0.1134 | 0.0895 |
Hour 4: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.1906 | 0.1708 | 0.0939 | 0.1434 | 0.1767 | 0.1029 | 0.1698 | 0.1784 | 0.0908 |
C1, R2 | 0.1923 | 0.1717 | 0.0953 | 0.1479 | 0.179 | 0.1045 | 0.1761 | 0.1805 | 0.0904 |
C1, R3 | 0.1951 | 0.1747 | 0.1072 | 0.1504 | 0.1809 | 0.1049 | 0.1741 | 0.184 | 0.0911 |
C1, R4 | 0.1996 | 0.1797 | 0.0979 | 0.1519 | 0.1833 | 0.109 | 0.1788 | 0.1824 | 0.0906 |
C2, R1 | 0.1889 | 0.1833 | 0.0961 | 0.1369 | 0.1802 | 0.0958 | 0.1703 | 0.1872 | 0.0902 |
C2, R2 | 0.1903 | 0.1844 | 0.0986 | 0.1368 | 0.1863 | 0.0988 | 0.171 | 0.1956 | 0.092 |
C2, R3 | 0.189 | 0.1891 | 0.0971 | 0.139 | 0.1821 | 0.0974 | 0.1701 | 0.1874 | 0.0958 |
C2, R4 | 0.1891 | 0.1865 | 0.0953 | 0.1417 | 0.1815 | 0.0976 | 0.167 | 0.1845 | 0.0895 |
Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.2852 | 0.2515 | 0.0937 | 0.2081 | 0.2876 | 0.1312 | 0.2516 | 0.2616 | 0.0902 |
C1, R2 | 0.2722 | 0.2545 | 0.0956 | 0.2188 | 0.2918 | 0.1308 | 0.2483 | 0.2663 | 0.0907 |
C1, R3 | 0.2719 | 0.2535 | 0.0956 | 0.2184 | 0.2929 | 0.1328 | 0.2542 | 0.2714 | 0.0912 |
C1, R4 | 0.2815 | 0.2591 | 0.0971 | 0.2148 | 0.2959 | 0.1347 | 0.2488 | 0.2649 | 0.0905 |
C2, R1 | 0.2634 | 0.2673 | 0.0954 | 0.1858 | 0.2583 | 0.102 | 0.2342 | 0.2635 | 0.0901 |
C2, R2 | 0.2651 | 0.2679 | 0.097 | 0.1857 | 0.2638 | 0.1042 | 0.2372 | 0.2719 | 0.0926 |
C2, R3 | 0.268 | 0.2681 | 0.0952 | 0.1875 | 0.2624 | 0.1047 | 0.2326 | 0.2628 | 0.0928 |
C2, R4 | 0.2525 | 0.2702 | 0.1024 | 0.1837 | 0.2582 | 0.1034 | 0.2384 | 0.2648 | 0.0901 |
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 63 | 69 | 130 | 83 | 64 | 76 | 66 | 64 | 64 |
C1, R2 | 63 | 72 | 129 | 84 | 64 | 75 | 70 | 65 | 64 |
C1, R3 | 64 | 70 | 131 | 84 | 65 | 76 | 66 | 66 | 67 |
C1, R4 | 64 | 72 | 132 | 84 | 65 | 77 | 67 | 65 | 65 |
C2, R1 | 63 | 71 | 124 | 81 | 63 | 74 | 68 | 65 | 66 |
C2, R2 | 63 | 71 | 122 | 82 | 63 | 74 | 65 | 65 | 68 |
C2, R3 | 64 | 71 | 123 | 82 | 64 | 75 | 67 | 63 | 67 |
C2, R4 | 64 | 71 | 123 | 83 | 64 | 75 | 65 | 65 | 66 |
Hour 2: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 67 | 112 | 135 | 135 | 70 | 97 | 94 | 65 | 65 |
C1, R2 | 68 | 113 | 139 | 134 | 71 | 98 | 96 | 67 | 67 |
C1, R3 | 66 | 113 | 142 | 137 | 69 | 97 | 98 | 68 | 66 |
C1, R4 | 66 | 115 | 136 | 139 | 71 | 95 | 95 | 65 | 70 |
C2, R1 | 65 | 122 | 133 | 128 | 67 | 86 | 95 | 66 | 70 |
C2, R2 | 64 | 121 | 136 | 129 | 66 | 84 | 97 | 65 | 65 |
C2, R3 | 63 | 118 | 133 | 131 | 66 | 86 | 97 | 65 | 66 |
C2, R4 | 63 | 117 | 136 | 129 | 67 | 86 | 98 | 64 | 65 |
Hour 4: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 67 | 170 | 153 | 235 | 76 | 118 | 126 | 70 | 65 |
C1, R2 | 69 | 173 | 150 | 236 | 77 | 116 | 128 | 71 | 66 |
C1, R3 | 68 | 179 | 156 | 237 | 76 | 117 | 125 | 71 | 66 |
C1, R4 | 69 | 178 | 155 | 235 | 76 | 121 | 128 | 70 | 67 |
C2, R1 | 66 | 175 | 145 | 216 | 70 | 91 | 124 | 72 | 67 |
C2, R2 | 69 | 176 | 143 | 209 | 72 | 88 | 124 | 69 | 65 |
C2,3 | 66 | 176 | 145 | 219 | 70 | 92 | 124 | 70 | 66 |
C2, R4 | 68 | 177 | 145 | 216 | 73 | 90 | 123 | 72 | 66 |
Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 72 | 256 | 154 | 385 | 85 | 147 | 161 | 77 | 65 |
C1, R2 | 72 | 263 | 158 | 388 | 88 | 146 | 161 | 76 | 66 |
C1, R3 | 71 | 266 | 158 | 395 | 87 | 149 | 164 | 77 | 66 |
C1, R4 | 73 | 272 | 160 | 379 | 84 | 149 | 160 | 73 | 67 |
C2, R1 | 72 | 259 | 148 | 325 | 78 | 93 | 148 | 74 | 66 |
C2, R2 | 71 | 258 | 147 | 321 | 77 | 96 | 152 | 75 | 64 |
C2, R3 | 70 | 259 | 146 | 323 | 79 | 97 | 151 | 76 | 66 |
C2, R4 | 69 | 259 | 143 | 315 | 79 | 97 | 146 | 75 | 66 |