Difference between revisions of "Team:XMU-China/Contribution"

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<span class="subtitle" id="subtitle2">-----* Experiment *-----</span>
 
<span class="subtitle" id="subtitle2">-----* Experiment *-----</span>
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<p>Firstly, we got the biobrick called <strong>K1357009</strong> from the <strong>2017 DNA Distribution Kit Plates</strong> and transformed it to the E.Coli DH5α. <strong>After a one-week incubation</strong>, a big bacterial colony grown on the plate.</p><br />
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<p>Firstly, we got <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> from the <strong>2017 DNA Distribution Kit Plates</strong> and transformed it to the E.Coli DH5α. <strong>After a one-week incubation</strong>, a big bacterial colony grown on the plate.</p><br />
 
<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/e/e9/T--XMU-China--contributionimg1.jpeg"></div><br /><br />
 
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<p>Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid J61002 with the promoter J23100, then transformed into E.coli DH5α again. We observed the <strong>strong blue just one day later</strong>. The result shows that BBa_K1357009 works well. </p><br />
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<p>Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid <a href="http://parts.igem.org/Part:BBa_J61002">BBa_J61002</a> with the promoter J23100, then transformed into E.coli DH5α again. We observed the <strong>strong blue just one day later</strong>. The result shows that BBa_K1357009 works well. </p><br />
 
<div class="contributionimg"><img class="contributionimg2" src="https://static.igem.org/mediawiki/2017/2/2a/T--XMU-China--contributionimg2.jpeg"></div>
 
<div class="contributionimg"><img class="contributionimg2" src="https://static.igem.org/mediawiki/2017/2/2a/T--XMU-China--contributionimg2.jpeg"></div>
 
<div class="contributionimg"><img class="contributionimg3" src="https://static.igem.org/mediawiki/2017/c/c1/T--XMU-China--contributionimg3.jpeg"></div>
 
<div class="contributionimg"><img class="contributionimg3" src="https://static.igem.org/mediawiki/2017/c/c1/T--XMU-China--contributionimg3.jpeg"></div>

Revision as of 12:57, 29 October 2017

2017.igem.org/Team:XMU-China/Contribution

-----* Introduce *-----

The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the coral Acropora millepora, amilCP. In this year, we want to verify the characteristic of BFP.

-----* Experiment *-----

Firstly, we got BBa_K1357009 from the 2017 DNA Distribution Kit Plates and transformed it to the E.Coli DH5α. After a one-week incubation, a big bacterial colony grown on the plate.




Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid BBa_J61002 with the promoter J23100, then transformed into E.coli DH5α again. We observed the strong blue just one day later. The result shows that BBa_K1357009 works well.