Difference between revisions of "Team:BOKU-Vienna/Notebook"

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<div id="2" class="yolo" style="display:none;">
 
<p>
 
<p>
−
<br>• PCR: 1, 2, 3, 4, 6, 15, 18, 20, 23, 24, 25, 26</br>
+
<br>• PCR: #1, #2, #3, #4, #6, #15, #18, #20, #23, #24, #25, #26</br>
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly: BB1_09, BB1_10, BB1_11, BB1_12, BB1_13, BB2_01 (unsuccessful), BB2_02, BB2_03, BB2_04, BB2_05 (unsuccessful)</br>
 
<br><br>• Golden Gate Assembly: BB1_09, BB1_10, BB1_11, BB1_12, BB1_13, BB2_01 (unsuccessful), BB2_02, BB2_03, BB2_04, BB2_05 (unsuccessful)</br>
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<div id="3" class="yolo" style="display:none;">
 
<p>
 
<p>
−
<br>• PCR: 27, 28</br>
+
<br>• PCR: #27, #28</br>
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly: BB1_14, BB1_15, BB2_01 (unsuccessful, even with new BBa_23101 promoter), BB2_05 (unsuccessful), BB2_06, BB2_07, BB2_08, BB2_09, BB2_10, BB2_12, BB2_14, BB2_15, BB2_16, C-PPP_01 (unsuccessful)</br>
 
<br><br>• Golden Gate Assembly: BB1_14, BB1_15, BB2_01 (unsuccessful, even with new BBa_23101 promoter), BB2_05 (unsuccessful), BB2_06, BB2_07, BB2_08, BB2_09, BB2_10, BB2_12, BB2_14, BB2_15, BB2_16, C-PPP_01 (unsuccessful)</br>
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<div id="4" class="yolo" style="display:none;">
 
<p>
 
<p>
−
<br>• PCR: 9, 10, 16, 29, 30, 31, 32, 33, 34, 35, 36, 41, 42, 43</br>
+
<br>• PCR: #9, #10, #16, #29, #30, #31, #32, #33, #34, #35, #36, #41, #42, #43</br>
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly: BB1_16, BB1_17, BB1_18, BB1_20, BB1_21, BB1_22, BB1_23, BB1_24, BB1_25, BB2_17, BB2_23, BB2_24, BB3_01 (unsuccessful), BB3_02, , BB3_04, BB3_05, BB3_06 (doubtful), BB3_08</br>
 
<br><br>• Golden Gate Assembly: BB1_16, BB1_17, BB1_18, BB1_20, BB1_21, BB1_22, BB1_23, BB1_24, BB1_25, BB2_17, BB2_23, BB2_24, BB3_01 (unsuccessful), BB3_02, , BB3_04, BB3_05, BB3_06 (doubtful), BB3_08</br>
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<div id="5" class="yolo" style="display:none;">
 
<div id="5" class="yolo" style="display:none;">
 
<p>
 
<p>
−
<br>• PCR: 41, 42, 45, 52, 53, 56, 57</br>
+
<br>• PCR: #41, #42, #45, #52, #53, #56, #57</br>
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly: BB1_19, BB2_19, BB2_25, BB2_26, BB2_27, BB2_28, BB2_29, BB2_30, BB2_31, BB2_32, BB2_33, BB2_34, BB3_01 (unsuccessful), BB3_02, BB3_03 (unsuccessful), BB3_06, BB3_10, BB3_13-15, BB3_24</br>
 
<br><br>• Golden Gate Assembly: BB1_19, BB2_19, BB2_25, BB2_26, BB2_27, BB2_28, BB2_29, BB2_30, BB2_31, BB2_32, BB2_33, BB2_34, BB3_01 (unsuccessful), BB3_02, BB3_03 (unsuccessful), BB3_06, BB3_10, BB3_13-15, BB3_24</br>

Revision as of 13:45, 29 October 2017

Notebook

V

Notebook.


All protocols we used for the methods can be found here: Protocol

For further information regarding our Golden Gate Assembly products, please refer to our part collection site. 

Week 1 (03/07-07/07)
   
Week 2 (10/07-14/07)
   
Week 3 (17/07-21/07)
   
Week 4 (24/07-28/07)
   
Week 5 (31/07-04/08)
   
Week 6 (07/08-11/08)
   
Week 7 (14/08-18/08)
   
Week 8 (21/08-25/08)
   
Week 9 (28/08-01/09)
   
Week 10 (04/09-08/09)
   
Week 11 (11/09-15/09)
   
Week 12 (18/09-22/09)
   
Week 13 (25/09-29/09)