Difference between revisions of "Team:XMU-China/Contribution"

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<span class="subtitle" id="subtitle1">-----* Introduce *-----</span>
 
<span class="subtitle" id="subtitle1">-----* Introduce *-----</span>
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<p>The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the <strong>coral Acropora millepora, amilCP</strong>. In this year, we want to verify the characteristic of BFP.</p>
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<p>We also improved the characterization of two existing BioBrick Parts although we've participated in <a href="https://2017.igem.org/Team:XMU-China/InterLab">the InterLab Measurement Study</a>. The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the <strong>coral Acropora millepora, amilCP</strong>. In this year, we want to verify the characteristic of BFP.</p>
 
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<span class="subtitle" id="subtitle2">-----* Experiment *-----</span>
 
<span class="subtitle" id="subtitle2">-----* Experiment *-----</span>

Revision as of 14:26, 29 October 2017

2017.igem.org/Team:XMU-China/Contribution

-----* Introduce *-----

We also improved the characterization of two existing BioBrick Parts although we've participated in the InterLab Measurement Study. The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the coral Acropora millepora, amilCP. In this year, we want to verify the characteristic of BFP.

-----* Experiment *-----

Firstly, we got BBa_K1357009 from the 2017 DNA Distribution Kit Plates and transformed it to the E.Coli DH5α. After a one-week incubation, a big bacterial colony grown on the plate.




Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid BBa_J61002 with the promoter J23100, then transformed into E.coli DH5α again. We observed the strong blue just one day later. The result shows that BBa_K1357009 works well.