Difference between revisions of "Team:XMU-China/Contribution"

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<span class="subtitle" id="subtitle1">-----* Introduce *-----</span>
 
<span class="subtitle" id="subtitle1">-----* Introduce *-----</span>
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<p>We also improved the characterization of two existing BioBrick Parts although we've participated in <a href="https://2017.igem.org/Team:XMU-China/InterLab">the InterLab Measurement Study</a>. The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the <strong>coral Acropora millepora, amilCP</strong>. In this year, we want to verify the characteristic of BFP.</p>
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<p>We also improved the characterization of two existing BioBrick Parts although we've participated in <a href="https://2017.igem.org/Team:XMU-China/InterLab">the InterLab Measurement Study</a>. The parts we tested are <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> and <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a>. </p>
 
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<span class="subtitle" id="subtitle2">-----* Experiment *-----</span>
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<span class="subtitle" id="subtitle2">-----* <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> *-----</span>
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<p>Firstly, we got <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> from the <strong>2017 DNA Distribution Kit Plates</strong> and transformed it to the E.Coli DH5α. <strong>After a one-week incubation</strong>, a big bacterial colony grown on the plate.</p><br />
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<p>The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the <strong>coral Acropora millepora, amilCP</strong>. In this year, we want to verify the characteristic of BFP. <br />Firstly, we got <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> from the <strong>2017 DNA Distribution Kit Plates</strong> and transformed it to the E.Coli DH5α. <strong>After a one-week incubation</strong>, a big bacterial colony grown on the plate.</p><br />
 
<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/e/e9/T--XMU-China--contributionimg1.jpeg"></div><br /><br />
 
<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/e/e9/T--XMU-China--contributionimg1.jpeg"></div><br /><br />
 
<p>Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid <a href="http://parts.igem.org/Part:BBa_J61002">BBa_J61002</a> with the promoter J23100, then transformed into E.coli DH5α again. We observed the <strong>strong blue just one day later</strong>. The result shows that BBa_K1357009 works well. </p><br />
 
<p>Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid <a href="http://parts.igem.org/Part:BBa_J61002">BBa_J61002</a> with the promoter J23100, then transformed into E.coli DH5α again. We observed the <strong>strong blue just one day later</strong>. The result shows that BBa_K1357009 works well. </p><br />

Revision as of 14:29, 29 October 2017

2017.igem.org/Team:XMU-China/Contribution

-----* Introduce *-----

We also improved the characterization of two existing BioBrick Parts although we've participated in the InterLab Measurement Study. The parts we tested are BBa_K1357009 and BBa_K540001.

-----* BBa_K1357009 *-----

The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the coral Acropora millepora, amilCP. In this year, we want to verify the characteristic of BFP.
Firstly, we got BBa_K1357009 from the 2017 DNA Distribution Kit Plates and transformed it to the E.Coli DH5α. After a one-week incubation, a big bacterial colony grown on the plate.




Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid BBa_J61002 with the promoter J23100, then transformed into E.coli DH5α again. We observed the strong blue just one day later. The result shows that BBa_K1357009 works well.