Difference between revisions of "Team:Oxford/Parts"
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| − | <td>BBa_K2450101</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2450101">BBa_K2450101</a></td> |
<td>C100</td> | <td>C100</td> | ||
<td>mCherry - TEV protease (non-cleaving)</td> | <td>mCherry - TEV protease (non-cleaving)</td> | ||
Revision as of 15:01, 29 October 2017
Parts
Parts for the Cell-Free System
| Registry Number | Code used in Lab | Brief Description | Key Details | Biobricks used | Other sequence sources |
|---|---|---|---|---|---|
| BBa_K2450101 | C100 | mCherry - TEV protease (non-cleaving) | Doesn’t self-cleave | ? | ? |
| BBa_K2450201 | C200 | Modified TetR with TEV cleavage site - CFP | TEV cleavage site in linker between DNA binding and dimerisation domains | BBa_K106669; BBa_K??????? | TEV cleavage site from Merck; CFP from AddGene |
| BBa_K2450251 | C250 | TetR - CFP | Normal TetR Class B | BBa_K106669; BBa_K??????? | CFP from AddGene |
| BBa_K2450301 | C300 | Tet promoter - RBS - YFP | DNA part, contains promoter and RBS to give signal of YFP | BBa_R0040 | ? |
Parts for the OMV System
| Registry Number | Code used in Lab | Brief Description | Key Details | Biobricks used | Other sequence sources |
|---|---|---|---|---|---|
| BBa_K2450401 | V100 | N-SpyCatcher - OmpA - sfGFP | SpyCatcher is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm | ? | Alves et al, 2016 AddGene |
| BBa_K2450451 | v150 | OmpA - C-SpyCatcher - sfGFP | SpyCatcher is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm | ? | Alves et al, 2016 |
| BBa_K2450501 | v200 | SpyTag - sfGFP - Quencher | TEV cleavage site between sfGFP and the quenching peptide | BBa_K1319014 | Alves et al, 2016 |
| BBa_K2450502 | V200noQ | SpyTag - sfGFP | Control to check localisation to OMVs | ? | Alves et al, 2016 |
| BBa_K2450701 | V500 | sfGFP - N-terminal split TEV Protease - Inhibited Coiled Coil | Inhibited coiled coil contains a TEV cleavage site between the two helicies, leaving the acid coiled coil attached to the N-terminal split protease | ? | Alves et al, 2016 Split TEV paper |
| BBa_K2450702 | V600 | mCherry - Inhibited Coiled Coil - C-terminal Split TEV Protease | Inhibited coiled coil contains a TEV protease cleavage site. This will result in the cleavage of the mCherry - Acid Coil being cleaved from the Base Coil - C Terminal Split TEV Protease. | ? | Alves et al, 2016 Split TEV paper |
Inspiration
We have a created a collection of well documented parts that can help you get started.
You can also take a look at how other teams have documented their parts in their wiki:
