Difference between revisions of "Team:Oxford/Parts"

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       </tr>
 
       </tr>
 
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     <td>BBa_K2450201</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_K2450201">BBa_K2450201</a></td>
 
     <td>C200</td>
 
     <td>C200</td>
 
     <td>Modified TetR with TEV cleavage site - CFP</td>
 
     <td>Modified TetR with TEV cleavage site - CFP</td>
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       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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      <td>BBa_K2450251</td>
+
      <td><a href="http://parts.igem.org/Part:BBa_K2450251">BBa_K2450251</a></td>
 
      <td>C250</td>
 
      <td>C250</td>
 
      <td>TetR - CFP</td>
 
      <td>TetR - CFP</td>
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  </tr>
 
  </tr>
 
  <tr>
 
  <tr>
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      <td>BBa_K2450301</td>
+
      <td><a href="http://parts.igem.org/Part:BBa_K2450301">BBa_K2450301</a></td>
 
      <td>C300</td>
 
      <td>C300</td>
 
      <td>Tet promoter - RBS - YFP</td>
 
      <td>Tet promoter - RBS - YFP</td>
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     <tbody>
 
     <tbody>
 
       <tr>
 
       <tr>
−
         <td>BBa_K2450401</td>
+
         <td><a href="http://parts.igem.org/Part:BBa_K2450401">BBa_K2450401</a></td>
 
         <td>V100</td>
 
         <td>V100</td>
 
         <td>N-SpyCatcher - OmpA - sfGFP</td>
 
         <td>N-SpyCatcher - OmpA - sfGFP</td>
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       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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     <td>BBa_K2450451</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_K2450451">BBa_K2450451</a></td>
 
     <td>v150</td>
 
     <td>v150</td>
 
     <td>OmpA - C-SpyCatcher - sfGFP</td>
 
     <td>OmpA - C-SpyCatcher - sfGFP</td>
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       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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      <td>BBa_K2450501</td>
+
      <td><a href="http://parts.igem.org/Part:BBa_K2450501">BBa_K2450501</a></td>
 
      <td>v200</td>
 
      <td>v200</td>
 
      <td>SpyTag - sfGFP - Quencher</td>
 
      <td>SpyTag - sfGFP - Quencher</td>
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  </tr>
 
  </tr>
 
  <tr>
 
  <tr>
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      <td>BBa_K2450502</td>
+
      <td><a href="http://parts.igem.org/Part:BBa_K2450502">BBa_K2450502</a></td>
 
      <td>V200noQ</td>
 
      <td>V200noQ</td>
 
      <td>SpyTag - sfGFP</td>
 
      <td>SpyTag - sfGFP</td>
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  </tr>
 
  </tr>
 
    <tr>
 
    <tr>
−
      <td>BBa_K2450701</td>
+
      <td><a href="http://parts.igem.org/Part:BBa_K2450701">BBa_K2450701</a></td>
 
      <td>V500</td>
 
      <td>V500</td>
 
      <td>sfGFP - N-terminal split TEV Protease - Inhibited Coiled Coil</td>
 
      <td>sfGFP - N-terminal split TEV Protease - Inhibited Coiled Coil</td>
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  </tr>
 
  </tr>
 
    <tr>
 
    <tr>
−
      <td>BBa_K2450702</td>
+
      <td><a href="http://parts.igem.org/Part:BBa_K2450702">BBa_K2450702</a></td>
 
      <td>V600</td>
 
      <td>V600</td>
 
      <td>mCherry - Inhibited Coiled Coil - C-terminal Split TEV Protease</td>
 
      <td>mCherry - Inhibited Coiled Coil - C-terminal Split TEV Protease</td>

Revision as of 15:04, 29 October 2017

Parts

Parts for the Cell-Free System

Registry Number Code used in Lab Brief Description Key Details Biobricks used Other sequence sources
BBa_K2450101 C100 mCherry - TEV protease (non-cleaving) Doesn’t self-cleave ? ?
BBa_K2450201 C200 Modified TetR with TEV cleavage site - CFP TEV cleavage site in linker between DNA binding and dimerisation domains BBa_K106669; BBa_K??????? TEV cleavage site from Merck; CFP from AddGene
BBa_K2450251 C250 TetR - CFP Normal TetR Class B BBa_K106669; BBa_K??????? CFP from AddGene
BBa_K2450301 C300 Tet promoter - RBS - YFP DNA part, contains promoter and RBS to give signal of YFP BBa_R0040 ?

Parts for the OMV System

Registry Number Code used in Lab Brief Description Key Details Biobricks used Other sequence sources
BBa_K2450401 V100 N-SpyCatcher - OmpA - sfGFP SpyCatcher is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm ? Alves et al, 2016 AddGene
BBa_K2450451 v150 OmpA - C-SpyCatcher - sfGFP SpyCatcher is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm ? Alves et al, 2016
BBa_K2450501 v200 SpyTag - sfGFP - Quencher TEV cleavage site between sfGFP and the quenching peptide BBa_K1319014 Alves et al, 2016
BBa_K2450502 V200noQ SpyTag - sfGFP Control to check localisation to OMVs ? Alves et al, 2016
BBa_K2450701 V500 sfGFP - N-terminal split TEV Protease - Inhibited Coiled Coil Inhibited coiled coil contains a TEV cleavage site between the two helicies, leaving the acid coiled coil attached to the N-terminal split protease ? Alves et al, 2016 Split TEV paper
BBa_K2450702 V600 mCherry - Inhibited Coiled Coil - C-terminal Split TEV Protease Inhibited coiled coil contains a TEV protease cleavage site. This will result in the cleavage of the mCherry - Acid Coil being cleaved from the Base Coil - C Terminal Split TEV Protease. ? Alves et al, 2016 Split TEV paper
Inspiration

We have a created a collection of well documented parts that can help you get started.

You can also take a look at how other teams have documented their parts in their wiki: