Difference between revisions of "Team:Baltimore Bio-Crew/HP/Silver"

Line 1: Line 1:
 
{{Baltimore_Bio-Crew}}
 
{{Baltimore_Bio-Crew}}
−
{{Team:Baltimore_Bio-Crew/JS}}
+
{{Team:Baltimore Bio-Crew/JS}}
 +
{{Team:Baltimore Bio-Crew/CSS}}
 +
{{Team:Baltimore Bio-Crew/templateReset}}
 +
{{Team:Baltimore Bio-Crew/basicCSS}}
 +
 
 
<html>
 
<html>
−
<div class="clear"></div>
 
  
 +
<style>
  
−
<div class="column full_size">
+
article{
 +
width: 50%;
 +
margin:0 auto;
  
−
<h1>Silver Medal Human Practices</h1>
+
}
−
<p>iGEM teams are leading in the area of Human Practices because they conduct their projects within a social/environmental context, to better understand issues that might influence the design and use of their technologies.</p>
+
article p{
−
<p>Teams work with students and advisors from the humanities and social sciences to explore topics concerning ethical, legal, social, economic, safety or security issues related to their work. Consideration of these Human Practices is crucial for building safe and sustainable projects that serve the public interest. </p>
+
font-size: 20px;
−
<p>For more information, please see the <a href="https://2017.igem.org/Competition/Human_Practices">Human Practices page</a>.</p>
+
font-style: italic;
−
</div>
+
padding-top: 10px;
 +
padding-bottom: 10px;
 +
font-family: 'Abel', san-serif;
 +
color: black;
  
−
<div class="clear"></div>
 
  
−
<div class="column half_size">
+
}
−
<h3>Silver Medal Criterion #3</h3>
+
.footerSection{
−
<p>Convince the judges you have thought carefully and creatively about whether your work is safe, responsible and good for the world. You could accomplish this through engaging with your local, national and/or international communities or other approaches. Please note that standard surveys will not fulfill this criteria.</p>
+
background-color:#a5f7c3;
−
</div>
+
      margin-top: 300px;
 +
}
  
−
<div class="column half_size">
+
footer{
−
<h5>Some Human Practices topic areas </h5>
+
width:60%;
−
<ul>
+
margin-left:35%;
−
<li>Philosophy</li>
+
}
−
<li>Public Engagement / Dialogue</li>
+
−
<li>Education</li>
+
−
<li>Product Design</li>
+
−
<li>Scale-Up and Deployment Issues</li>
+
−
<li>Environmental Impact</li>
+
−
<li>Ethics</li>
+
−
<li>Safety</li>
+
−
<li>Security</li>
+
−
<li>Public Policy</li>
+
−
<li>Law and Regulation</li>
+
−
<li>Risk Assessment</li>
+
−
</ul>
+
−
</div>
+
  
 +
.html{
 +
  height: 100%;
 +
}
 +
.Intro{
 +
  width: 100%;
 +
  height:100%;
 +
  margin: 0 0 0 0;
 +
}
 +
 +
h1{
 +
  font-size: 100px;
 +
  text-align: center;
 +
  color: black;
 +
  font-family: 'Saira', sans-serif;
 +
  padding-top: 40px;
 +
}
 +
h3{
 +
  font-size: 25px;
 +
  font-style: italic;
 +
  text-align: center;
 +
  padding-top: 20px;
 +
  padding-bottom: 30px;
 +
  font-family: 'Abel', san-serif;
 +
  font-style: italic;
 +
  color: black;
 +
text-decoration: underline;
 +
}
 +
h4{
 +
  font-size: 18px;
 +
  font-style: italic;
 +
  text-align: center;
 +
  padding-top: 20px;
 +
  padding-bottom: 30px;
 +
  font-family: 'Abel', san-serif;
 +
  color: black;
 +
}
  
−
<div class="column half_size">
 
−
<h5>What should we write about on this page?</h5>
 
−
<p>On this page, you should write about the Human Practices topics you considered in your project, and document any special activities you did (such as visiting experts, talking to lawmakers, or doing public engagement). This should include all of the work done for the Silver Medal Criterion #3. Details for your Gold medal work and/or work for the two Human Practices special prizes should be put on those specified pages.</p>
 
−
</div>
 
  
 +
h2{
 +
  padding: 75px 0 0 0;
 +
  font-size: 36px;
 +
  font-style: normal;
 +
  font-family: 'Saira', sans-serif;
 +
  text-align: center;
 +
color: black;
 +
}
  
−
<div class="column half_size">
+
 
−
<h5>Inspiration</h5>
+
 
−
<p>Read what other teams have done:</p>
+
</style>
−
<ul>
+
     
−
<li><a href="https://2014.igem.org/Team:Dundee/policypractice/experts">2014 Dundee </a></li>
+
      </head>
−
<li><a href="https://2014.igem.org/Team:UC_Davis/Policy_Practices_Overview">2014 UC Davis </a></li>
+
      <body>
−
<li><a href="https://2013.igem.org/Team:Manchester/HumanPractices">2013 Manchester </a></li>
+
<section>
−
<li><a href="https://2013.igem.org/Team:Cornell/outreach">2013 Cornell </a></li>
+
<header>
−
</ul>
+
        <div class="Intro">
 +
          <h1>BALTIMORE BIO-CREW</h1>
 +
          <h4>Bio-Engineering E.Coli To Degrade Plastic and Save The Baltimore Inner Harbor</h4>
 
</div>
 
</div>
 +
<hr>
 +
</header>
 +
</section>
 +
<section id="description" class= "projectDescription">
 +
<header>
 +
 +
                        <h3> About Our Project </h3>
 +
</header>
 +
<article>
 +
<p>
 +
Our goal for this project is to genetically engineer E. coli bacteria that can break down plastic. These bacteria could have many different applications, such as: degrading plastic waste from labs that cannot be recycled, being used in a filter to catch and degrade micro plastic fibers from laundry, and breaking down plastic in a marine environment into harmless molecules. We made a lot of progress last year, and this year we plan to build on that progress.
 +
</p>
 +
 +
<p>
 +
While searching for solutions to the issue of plastic pollution in the Baltimore Inner Harbor, we found a paper by Yoshida et. al. describing a bacteria called Ideonella sakaiensis that was capable of degrading PET plastic into monomers. The bacteria used the enzyme PETase (chlorogenate esterase) to break down PET into MHET, and the enzyme MHETase (Lipase) to break down MHET into ethylene glycol and therephthalic acid. We decided to use the genes from this bacteria for our project.
 +
</p>
 +
 +
<p>
 +
To avoid the safety risks of working with a relatively undocumented bacteria, we decided to take the plastic degradation genes from I. sakaiensis and put them into K12 E. coli bacteria. We chose E. coli because they are safe to work with and commonly used in the lab. Using the genetic sequence found in the paper, we designed the two plastic degrading enzymes so that they could be expressed in E. coli bacteria. We then had them synthesized and worked on putting these genes into E. coli.
 +
</p>
 +
 +
<p>
 +
By the end of last year’s competition, we had managed to insert the lipase gene into E.coli, but not the chlorogenate esterase gene. We confirmed that we had correctly inserted the lipase gene using colony PCR and gene sequencing, but we did not have the time to conduct additional assays, such as protein gels, to determine if the enzyme was being secreted from the bacteria. This year, we plan to redesign the chlorogenate esterase and lipase genes so that they contain the proper tags that will allow them to be detected, and a secretion sequence. After we insert both genes into E. coli cells, we will test them to make sure they can secrete the plastic degrading enzymes and degrade PET plastic.
 +
 +
 +
 +
</article>
 +
</section>
 +
 +
<section id="Footer" class="footerSection">
 +
<hr>
 +
 +
<h2>
 +
Sponsers
 +
</h2>
 +
<h4>
 +
The Baltimore Bio-Crew thanks our sponsors for their generous support of our team that made our project and travel to the Jamboree possible. Thank you!
 +
</h4>
 +
 +
<footer>
 +
 +
<a href="http://www.bd.com/en-us">
 +
  <img src="https://upload.wikimedia.org/wikipedia/en/f/f8/Update_Color_BD_PNG_Logo.png" alt="BD Medical Technology, Advancing the World of Health - BD" style="width:100px; height:100px;">
 +
</a>
 +
<a href="http://familyleague.org/">
 +
  <img src="http://baltimoreattendance.org/wp-content/uploads/2015/08/flbcinc-360x230.png" alt="Family League of Baltimore" style="width:100px; height:100px;">
 +
</a>
  
 +
<a href="https://www.rwdfoundation.org/">
 +
  <img src="https://static.igem.org/mediawiki/2016/6/65/T--Baltimore_BioCrew--DeutschFoundation.png" alt="The Robert W. Deutsch Foundation" style="width:100px; height:100px;">
 +
</a>
  
 +
<a href="http://vwrfoundation.org/">
 +
  <img src="https://static.igem.org/mediawiki/2016/1/1a/T--Baltimore_Biocrew--VWR_Foundation_LOGO.jpeg" alt="VWR Charitable Foundation" style="width:100px; height:100px;">
 +
</a>
 +
<a href="http://vwrfoundation.org/">
 +
  <img src="https://media.licdn.com/mpr/mpr/shrink_200_200/AAEAAQAAAAAAAAI8AAAAJDY0ZDg0ZjlkLWVlMTItNGI1Mi1iNWEwLWYzMDVlYWMwMTZhZg.png" alt="Maryland Recycling Network" style="width:100px; height:100px;">
 +
</a>
  
 +
</footer>
 +
</section>
 +
      </body>
 
</html>
 
</html>

Revision as of 18:02, 29 October 2017


BUGSS Logo
Home Team Project Results Design Notebook Composite Part Safety HP Silver Experiment Attribution Collaborations Interlab Contributions

.html{ height: 100%; } h1{ font-size: 100px; text-align: center; color: black; font-family: 'Saira', sans-serif; padding-top: 40px; text-shadow: 4px 4px #3bb1e2; } h3{ font-size: 25px; font-style: italic; text-align: center; text-shadow: 2px 2px #33FF33; padding-top: 20px; padding-bottom: 30px; font-family: 'Abel', san-serif; font-style: italic; color: black; text-decoration: underline; } h4{ font-size: 18px; font-style: italic; text-align: center; padding-top: 20px; padding-bottom: 30px; font-family: 'Abel', san-serif; color: black; } h2{ padding: 75px 0 0 0; font-size: 36px; font-style: normal; font-family: 'Saira', sans-serif; text-align: center; color: black; }

BALTIMORE BIO-CREW

Bio-Engineering E.Coli To Degrade Plastic and Save The Baltimore Inner Harbor

About Our Project

Our goal for this project is to genetically engineer E. coli bacteria that can break down plastic. These bacteria could have many different applications, such as: degrading plastic waste from labs that cannot be recycled, being used in a filter to catch and degrade micro plastic fibers from laundry, and breaking down plastic in a marine environment into harmless molecules. We made a lot of progress last year, and this year we plan to build on that progress.

While searching for solutions to the issue of plastic pollution in the Baltimore Inner Harbor, we found a paper by Yoshida et. al. describing a bacteria called Ideonella sakaiensis that was capable of degrading PET plastic into monomers. The bacteria used the enzyme PETase (chlorogenate esterase) to break down PET into MHET, and the enzyme MHETase (Lipase) to break down MHET into ethylene glycol and therephthalic acid. We decided to use the genes from this bacteria for our project.

To avoid the safety risks of working with a relatively undocumented bacteria, we decided to take the plastic degradation genes from I. sakaiensis and put them into K12 E. coli bacteria. We chose E. coli because they are safe to work with and commonly used in the lab. Using the genetic sequence found in the paper, we designed the two plastic degrading enzymes so that they could be expressed in E. coli bacteria. We then had them synthesized and worked on putting these genes into E. coli.

By the end of last year’s competition, we had managed to insert the lipase gene into E.coli, but not the chlorogenate esterase gene. We confirmed that we had correctly inserted the lipase gene using colony PCR and gene sequencing, but we did not have the time to conduct additional assays, such as protein gels, to determine if the enzyme was being secreted from the bacteria. This year, we plan to redesign the chlorogenate esterase and lipase genes so that they contain the proper tags that will allow them to be detected, and a secretion sequence. After we insert both genes into E. coli cells, we will test them to make sure they can secrete the plastic degrading enzymes and degrade PET plastic.