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<div><b>SpeechRepublic offers workshops and programs for the development of communication and presentation skills.</b> There are three elements at the center of their methods: being self-confident, inspiring others and having the courage to bring your message across. They offered our team a free workshop on presenting for four team members. This helps us greatly in preparing for our upcoming presentations, specifically the presentation at the Jamboree in Boston.</div>
 
<div><b>SpeechRepublic offers workshops and programs for the development of communication and presentation skills.</b> There are three elements at the center of their methods: being self-confident, inspiring others and having the courage to bring your message across. They offered our team a free workshop on presenting for four team members. This helps us greatly in preparing for our upcoming presentations, specifically the presentation at the Jamboree in Boston.</div>
 
<div><b>New England BioLabs is a company that develops and produces reagents for life sciences research.</b> They offer a wide range of products for molecular biology. They have been generous to many iGEM teams over the years, and supported our team as well this year with a DNA assembly kit.</div>
 
<div><b>New England BioLabs is a company that develops and produces reagents for life sciences research.</b> They offer a wide range of products for molecular biology. They have been generous to many iGEM teams over the years, and supported our team as well this year with a DNA assembly kit.</div>
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<div><b>The Utrecht Universiteitsfonds is an institution that supports Utrecht University's educational and research activities.</b> It offers grants to students and alumni for various activities. They sponsored our project with financial aid.</div>
 
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<div class="page-heading">Collaborations</div>
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For our project, we collaborated with other iGEM teams as well as Dutch institutes. The aim of these collaborations was to share knowledge, to improve our project or to make people more aware of synthetic biology in general.
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<br><br>
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<h2 class="subhead" id="subhead-2">Wageningen UR 2017 team (Mantis)</h2>
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Our team collaborated with the Wageningen team to obtain independent validations of both our and their biobricks.
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<br><br>
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Wageningen validated the secreted-Cas9 and secreted-Cpf1 biobricks for us (BB_numbers). Members of the Wageningen team came to Utrecht to perform this validation together with members of the Utrecht team in our lab. Hek293T-cells were transfected with secreted-Cas9 or secreted-Cpf1 or with regular intracellular Cas9 or Cpf1 as a control. Medium of the cells was analysed on a western blot to compare extracellular protein expression. This tells us whether Cas9 and Cpf1 are truly being secreted or if they just leak out of the cells. The results of this validation can be found on the Parts Page (> Links to [Basic Parts]) and the experimental page on secreted Cas9 and Cpf1. (>Links to [Experimental][Secreted])
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<br><br>
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In return, for the Wageningen team, we did a blind validation of some of their biobricks. We performed a CpxR BiFC measurement, where E.coli K12 cells were transformed with the pSB1C3-araCpBAD-CpxReYFPn-CpxReYFPc plasmid. Fluorescence was then measured over the course of 6 hours.(>Links to [Wageningen Collab Page]) We also performed a leucine zipper BiFC measurement, where we transformed split sfGFP and split Venus to E.coli K12 and also full sfGFP and full Venus as controls. Cells were then lysed and fluorescence was measured in the cell lysate. (>Links to [Wageningen Collab Page])
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<br><br>
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<b>NICE PICTURE OF US AND WAGENINGEN GUYS IN SOME LAB</b>
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<br><br>
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<h2 class="subhead" id="subhead-3">RIVM (National Institute for Public Health and the Environment)</h2>
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As safety is a very important aspect in synthetic biology, we collaborated with the RIVM National Institute for Public Health and Environment of the Netherlands. They encouraged us to think about safety for different stakeholders. The information we gathered was summarized in an infographic, which we used to talk to the general public about synthetic biology and safety at an event organized by the RIVM. (>Links to [Human Practices][Safety])
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<b>NICE PICTURE OF RAWAN AND LISHI AT THE RIVM</b>
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<br><br>
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<h2 class="subhead" id="subhead-4">Dutch national science platform "De Kennis van Nu"</h2>
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Besides working in the lab on creating a diagnostic tool, we also tried to reach out to the public to make our project (and science in general) accessible and understandable to everyone. To achieve this, we collaborated with ‘de Kennis van Nu’, a Dutch platform of the national public broadcasting organization, that brings different scientific themes to the general public in an understandable way. On this platform we explain the formation of Utrecht’s first team, our design and how we are trying to solve healthcare problems. More information and some of the short videos we made can be found on our Outreach page (>Links to [Human Practices][Outreach]) and on the website of “De Kennis van Nu”
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(>https://www.dekennisvannu.nl/site/special/iGEM-2017-studenten-ontwerpen-nieuw-leven/111).
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<br><br>
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<h2 class="subhead" id="subhead-5">Düsseldorf Cologne postcards campaign</h2>
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To bring synthetic biology to the attention of the general public, we contributed a postcard to the Düsseldorf Cologne postcards campaign. Each team participating in this campaign designed a postcard including information about synthetic biology in general and about the different iGEM projects. These postcards will be sent to a large number of people in order to improve their perception of genetic engineering and synthetic biology.
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<b>PICTURE OF POSTCARDS LISHI IS GONNA MAKE</b>
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3: "RIVM (National Institute for Public&hellip;",
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4: "Dutch national science platform&hellip;",
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5: "Düsseldorf Cologne postcards camp&hellip;"
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},
 
"achievements" : {
 
"achievements" : {
 
1: "Bronze medals",
 
1: "Bronze medals",

Revision as of 18:39, 29 October 2017

<!DOCTYPE html>

  • Home
  • Experimental
  • Human practices
  • Team & Sponsors
Cas9 & Cpf1 secretion
and activity
Comparison of endonuclease activity for Cas9 and Cpf1 that has been produced in, and excreted by, HEK293 cells.
MESA two-component system replication
Details on the MESA two-component system, explanation of its relation to our design and the results of its reproduction.
OUTCASST system production
Detailed explanation of the OUTCASST mechanism, experimental progress and technical prospects.
Modeling and
mathematics
Ordinary differential equations, cellular automaton and an object based model for optimal linker-length estimation.
InterLab study participation
Results and details of our measurements for the iGEM 2017 InterLab Study.
Stakeholders & opinions
Interviews and dialogues with stakeholders, potential users, third parties and experts relating to pathogen detection or DNA-based diagnostics.
Risks & safety-issues
Implications and design considerations relating to safety in the usage and implementation of OUTCASST as a diagnostics tool.
Design & integration
OUTCASST toolkit and product design with factors such as bio-safety and user-friendliness taken into account.
Outreach
.
Meet our team
About us, our interests and roles in the team and our supervisors.
Sponsors
A listing of our sponsors, how they assisted us and our gratitude for their assistance.
Collaborations
.
Achievements
A short description of all that we have achieved during our participation in the iGEM.
Intro
The problem
The system