Difference between revisions of "Team:Bristol/Safety"

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<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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        <h1 class="Up">Safety</h1>
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        <p class="Up">In Development</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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      <div class="col-md-6 Up">
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        <h2>Safety features of our project design</h2>
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        <p>
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          The real world implementation of our BREATHE pods provides a unique situation where the general public will be walking next to cultures of E. coli during their day to day activities. The street-side location of our pods makes them particularly vulnerable
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          to damage, from vandalism, traffic incidents and extreme weather to name a few examples. It is therefore our responsibility to reduce the risk of the general public coming into contact with the E. coli inside our pods, and to reduce the harm
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          done if this does occur.
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        </p>
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        <p>
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          Using lab-dependent Top10 cells provides a primary level of protection.
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          <h2>Safety features of our project design</h2>
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          <div class="col-md-7 Up">
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            <p class="lead">
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              The real world implementation of our BREATHE pods provides a unique situation where the general public will be walking next to cultures of E. coli during their day to day activities. The street-side location of our pods makes them particularly vulnerable to damage, from vandalism, traffic incidents and extreme weather to name a few examples. It is therefore our responsibility to reduce the risk of the general public coming into contact with the E. coli inside our pods, and to reduce the harm done if this does occur.
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              <br><br>Using lab-dependent Top10 cells provides a primary level of protection.
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            </p>
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          </div>
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        <div class="col-md-5 Pop">
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          <img class="featurette-image img-responsive center-block" src="https://static.igem.org/mediawiki/2017/5/50/T--Bristol--Pods.png">
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        </div>
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<h5>Safe Project Design</h5>
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        <p class="lead">
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          Our pods should:
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        </p>
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        <p class="lead">
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          <ul class="list-group">
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            <li class="list-group-item">&#8226; Be leak proof</li>
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            <li class="list-group-item">&#8226; Be shatter-proof</li>
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            <li class="list-group-item">&#8226; Be impact-resistant</li>
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            <li class="list-group-item">&#8226; Have nearby hand sanitizer dispensers</li>
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            <li class="list-group-item">&#8226; Be high up to reduce risk of impact (where this would not severely impair functionality)</li>
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          </ul>
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        </p>
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        <h3>[image title]</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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          <div class="row parts align-items-center fadeIn">
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            <div class="col-md-12 Up">
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              <h2>Safety in the lab</h2>
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            </div>
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            <div class="col-md-9 Right notebook">
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              <p class="lead">
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                The following safety procedures were followed in the lab:
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              </p>
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              <ul class="list-group lead">
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                <li class="list-group-item">&#8226; Ethidium bromide was handled only when wearing gloves. All ethidium bromide waste was disposed of in sealed containers or in specific bins.</li>
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                <li class="list-group-item">&#8226; Any glassware which touched ethidium bromide was rinsed, and the water detoxified.</li>
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                <li class="list-group-item">&#8226; Suitable PPE was worn at all times when handling molecular biology reagents: lab coat, gloves, closed shoes.</li>
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                <li class="list-group-item">&#8226; All work handling E. coli was conducted using aseptic technique.</li>
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                <li class="list-group-item">&#8226; Gel extraction for purification of PCR products was conducted in a dark room, wearing UV resistant eye protection and with all skin covered.</li>
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              </ul>
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            </div>
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              <h3>[image title]</h3>
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          </div>
  
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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        <h2>Safe shipment</h2>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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</div>
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      <div class="col-md-9 Right notebook">
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        <p>
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          Text
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        </p>
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          <p class="lead">
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            <ul class="list-group">
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              <li class="list-group-item">&#8226; Text</li>
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              <li class="list-group-item">Text</li>
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              <li class="list-group-item">Text</li>
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            </ul>
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Revision as of 19:10, 29 October 2017

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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

Safety features of our project design

The real world implementation of our BREATHE pods provides a unique situation where the general public will be walking next to cultures of E. coli during their day to day activities. The street-side location of our pods makes them particularly vulnerable to damage, from vandalism, traffic incidents and extreme weather to name a few examples. It is therefore our responsibility to reduce the risk of the general public coming into contact with the E. coli inside our pods, and to reduce the harm done if this does occur.

Using lab-dependent Top10 cells provides a primary level of protection.

Our pods should:

  • • Be leak proof
  • • Be shatter-proof
  • • Be impact-resistant
  • • Have nearby hand sanitizer dispensers
  • • Be high up to reduce risk of impact (where this would not severely impair functionality)

Safety in the lab

The following safety procedures were followed in the lab:

  • • Ethidium bromide was handled only when wearing gloves. All ethidium bromide waste was disposed of in sealed containers or in specific bins.
  • • Any glassware which touched ethidium bromide was rinsed, and the water detoxified.
  • • Suitable PPE was worn at all times when handling molecular biology reagents: lab coat, gloves, closed shoes.
  • • All work handling E. coli was conducted using aseptic technique.
  • • Gel extraction for purification of PCR products was conducted in a dark room, wearing UV resistant eye protection and with all skin covered.
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