Attributions

We are proud to say that all the ideas we worked on this year were proposed by undergraduate members of iGEM 2017 team TokyoTech. We also want to thank all those advisors and instructors who helped us discuss these ideas and guided us through doing the experiments. The whole experimental data produced by our team comes from experiments all carried out by undergraduates.

The project "Coli Sapiens" was proposed by Hazuki Hasegawa and Hajime Fujita.

Members

Performing all cloning (transformation, culture cells, mini prep)

- Hazuki Hasegawa, Kazunori Motai, Takuma Yasue, Kohei Umedera, Saki Arakaki

Performing parts construction (restriction digest, ligation)

- Hazuki Hasegawa, Kazunori Motai, Takuma Yasue, Kohei Umedera, Saki Arakaki

Performing TraI Assay

- Kazunori Motai

Performing Human Cell Assay

- Saki Arakaki, Takuma Yasue

Performing AHK4 assay

- Kohei Umedera

Modeling

- Michio Takagi, Saki Arakaki, Hajime Fujita, Hazuki Hasegawa

Wiki and slide design

- Hajime Fujita, Hazuki Hasegawa, Moe Takahashi, Kazu Isawa with help from other team members

Human Practices

- Hajime Fujita, Moe Takahashi, Kazu Isawa, Hinako Kataoka, Hikaru Nakaya, Tamon Sato with help from other team members

Contributions

• General Support

  - Prof. Nakashima and Prof. Hayashi let us use their labs for all of our experiments and gave us advice on experimental skills.

  - Prof. Tagawa gave us advice on our project and helped us set up a forum.

  - Prof. Yamamura gave us advice on modeling.

  - Promega Corporation and Leave a Nest Co.,ltd gave us several kinds of restriction enzymes used in our experiments at no charge.

  - We'd like to thank all advisors and instructors who helped us through the discussion of these ideas and instructed us about experimental procedures.




• Instrumental Analysis Support

  - Biomaterial Analysis Center, Technical Department, checked the sequences of the plasmids which we asked.




• Gene Synthesis

  - We'd also like to thank MBL and IDT.




• Human Practice Support

  - Koshigaya-Kita High School and Kasukabe High School warmly accepted our school visits.

  - Prof. Kiga, Prof. Oshima, Dr. Jiro Nudeshima, who are working on The Tokyo Foundation, gave us lectures.

  - Prof. Nakasaki gave us great and helpful advice.




• Presentation Advisor

  - We'd like to thank all the people who came to check our presentations and gave us useful feedbacks:

  Professor:

  Yasunori Aizawa, Noriyuki Asakura, Hiroshi Iwasaki, Toshiaki Kamachi, Yasunori Tanji, Takeshi Hata, Takeshi Hirasawa, Junji Hirota, Shinji Masuda, Yuki Yamaguchi, Hideya Yuasa, Masaaki Wachi, Robert F. Whittier