Difference between revisions of "Team:CU-Boulder/Isolation"
| Line 185: | Line 185: | ||
<div class = "middleboxContainer"> | <div class = "middleboxContainer"> | ||
<div class = "middleboxVert"> | <div class = "middleboxVert"> | ||
| + | <div class = "dummyParagraph"> | ||
| + | <div class = "dummyGraphOneTitle"> | ||
<div class = "dummyGraphContainer"> | <div class = "dummyGraphContainer"> | ||
<div class = "dummyGraph"> | <div class = "dummyGraph"> | ||
| Line 193: | Line 195: | ||
</div> | </div> | ||
</div> | </div> | ||
| − | + | </div> | |
| + | </div> | ||
<div class = "middleboxVert"> | <div class = "middleboxVert"> | ||
| + | <div class = "dummyParagraph"> | ||
| + | |||
| + | <div class = "dummyGraphOneTitle"> | ||
| + | <h>Well Layout </h> | ||
| + | |||
| + | </div> | ||
| + | |||
<div class = "dummyGraphContainer"> | <div class = "dummyGraphContainer"> | ||
<div class = "dummyGraph"> | <div class = "dummyGraph"> | ||
| − | <img src = "https://static.igem.org/mediawiki/2017/ | + | <img src = "https://static.igem.org/mediawiki/2017/e/e7/T-CU_Boulder--PlatePattern.jpeg"/> |
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 23:10, 29 October 2017
Bacterial microcompartments are protein-based organelles that can enclose enzymes and other proteins, however without successful isolation of the compartments they do not have much use. We used methods previously described [1] to isolate the microcompartments through lysis and ultracentrifugation then confirmed isolation of the micro compartments in two ways: 1. Fluorescent foci (Fusion Red-LVA and Fusion Red Luciferase LVA) to identify the compartments in fluorescent imaging. With Eut-C tags, the fluorescent markers accumulate inside the compartment, allowing them to be clearly visible inside the compartment. The LVA protein degradation tag targets any excess fluorescent proteins for degradation to allow a sufficient background for viewing the compartments in vivo.
