Difference between revisions of "Team:MIT/2mk-HBG"
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<h1 style="color:#f20253; text-align: center; font-size: 40px; line-height: 40px;">Experiments with 2 Exon mKate HBG Reporter</h1> | <h1 style="color:#f20253; text-align: center; font-size: 40px; line-height: 40px;">Experiments with 2 Exon mKate HBG Reporter</h1> | ||
| + | <p>We designed a reporter similar to the mKate FF4 reporter, except the intron has been changed.</p> | ||
| + | <p>The mKate HBG reporter features the red fluorescent mKate gene split into two exons, with human beta globin (HBG) intron 2 in between these two exons. We used this split mKate 2-exon construct as a reporter construct that we used to determine if our dCas13 or Ms2 systems were successful in splicing out the HBG Intron 2. We used HBG Intron 2 for reasons outlined in our intron design discussion.Upstream of these sequences is a either the constitutive hEF1a promoter or the DOX controlled TRE-tight promoter. </p> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/0/03/2ex.png"> | ||
</html> | </html> | ||
Revision as of 00:57, 30 October 2017
Experiments with 2 Exon mKate HBG Reporter
We designed a reporter similar to the mKate FF4 reporter, except the intron has been changed.
The mKate HBG reporter features the red fluorescent mKate gene split into two exons, with human beta globin (HBG) intron 2 in between these two exons. We used this split mKate 2-exon construct as a reporter construct that we used to determine if our dCas13 or Ms2 systems were successful in splicing out the HBG Intron 2. We used HBG Intron 2 for reasons outlined in our intron design discussion.Upstream of these sequences is a either the constitutive hEF1a promoter or the DOX controlled TRE-tight promoter.
