Revision as of 02:42, 30 October 2017

April

2017.04.01	HP	Communicated with one of the authors of the article "Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation" online to get some latest progress.
2017.04.08	Attempts in E.coli	Got the Nitratireductor pacificus which contain our gene rdhANP.
2017.04.10	Attempts in E.coli	Started to culture N. pacificus.
2017.04.11	Attempts in E.coli	Extracted whole genome of N. pacificus.
2017.04.12	Attempts in E.coli	Got our gene rdhANP by PCR
2017.04.21	Attempts in Bacillus subtilis	Amplified rdhANP by colony PCR of N. pacificus
2017.04.25	Preparation of the protoplast for Bacillus megaterium	Made protoplast of B.megaterium
2017.04.26	Attempts in B. subtilis	Amplified rdhANP by colony PCR of N. pacificus
2017.04.26	Preparation of the protoplast for B.megaterium	Prepared prot-medium
2017.04.27	Attempts in B. subtilis	Agarose gel electrophoresis confirmed that the gene rdhANP is successfully amplified
2017.04.27	Preparation of the protoplast for B.megaterium	Prepared hyp-medium
2017.04.29	Attempts in E.coli	Amplified rdhANP by colony PCR of N. pacificus
2017.04.30	Attempts in E.coli	Cultured the E.coli with plasmid pET28a+

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 <table border="1" align="center">
-	<tr><td style="text-align:center;vertical-align:middle;">2017.04.01</td>
+	<tr><td style="width:100;text-align:center;vertical-align:middle;">2017.04.01</td>
 	<td style="vertical-align:middle;">HP</td>
 	<td style="vertical-align:middle;">Communicated with one of the authors of the article "Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation"  online to get some latest progress.</td></tr>