Difference between revisions of "Team:WHU-China/Notebook"
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| − | <tr><td style=" | + | <tr><td style="text-align:center;vertical-align:middle;">2017.04.01</td> |
| − | <td style=" | + | <td style="vertical-align:middle;">HP</td> |
| − | <td style=" | + | <td style="vertical-align:middle;">Communicated with one of the authors of the article "Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation" online to get some latest progress.</td></tr> |
<tr><td style="text-align:center;vertical-align:middle;">2017.04.08</td> | <tr><td style="text-align:center;vertical-align:middle;">2017.04.08</td> | ||
<td style="vertical-align:middle;">Attempts in E.coli</td> | <td style="vertical-align:middle;">Attempts in E.coli</td> | ||
Revision as of 02:43, 30 October 2017
April
| 2017.04.01 | HP | Communicated with one of the authors of the article "Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation" online to get some latest progress. |
| 2017.04.08 | Attempts in E.coli | Got the Nitratireductor pacificus which contain our gene rdhANP. |
| 2017.04.10 | Attempts in E.coli | Started to culture N. pacificus. |
| 2017.04.11 | Attempts in E.coli | Extracted whole genome of N. pacificus. |
| 2017.04.12 | Attempts in E.coli | Got our gene rdhANP by PCR |
| 2017.04.21 | Attempts in Bacillus subtilis | Amplified rdhANP by colony PCR of N. pacificus |
| 2017.04.25 | Preparation of the protoplast for Bacillus megaterium | Made protoplast of B.megaterium |
| 2017.04.26 | Attempts in B. subtilis | Amplified rdhANP by colony PCR of N. pacificus |
| 2017.04.26 | Preparation of the protoplast for B.megaterium | Prepared prot-medium |
| 2017.04.27 | Attempts in B. subtilis | Agarose gel electrophoresis confirmed that the gene rdhANP is successfully amplified |
| 2017.04.27 | Preparation of the protoplast for B.megaterium | Prepared hyp-medium |
| 2017.04.29 | Attempts in E.coli | Amplified rdhANP by colony PCR of N. pacificus |
| 2017.04.30 | Attempts in E.coli | Cultured the E.coli with plasmid pET28a+ |
