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<p>Hiroe, A., Tsuge, K., Nomura, C.T., Itaya, M. & Tsuge, T. (2012). Rearrangement of Gene Order in the phaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli. Appl. Environ. Microbiol., 78(9): 3177-3184</p>
 
<p>Hiroe, A., Tsuge, K., Nomura, C.T., Itaya, M. & Tsuge, T. (2012). Rearrangement of Gene Order in the phaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli. Appl. Environ. Microbiol., 78(9): 3177-3184</p>

Revision as of 03:06, 30 October 2017

Header

Judging Medal Requirements

BRONZE MEDAL

Register and Attend

The team has registered and will be attending the 2017 Giant Jamboree Boston from November 9th-13th!

Deliverables

  • Wiki
  • Poster
  • Presentation
  • Safety Forms
  • Judging Form
  • Registry Part Pages
  • Sample Submissions

Attributions

Check out our Attributions page for detailed information concerning the contributions of team members, mentors, and advisors!

Characterization/Contribution

Our InterLab page shows how our team participated in the InterLab study this year!

SILVER MEDAL

Validate a Part

BBa_K2260001

We submitted a new gene to the registry to assist in the production of PHB: the phaJ gene that codes for PHB enoyl-CoA hydratase. This part contains both the phaJ and phaC genes and was shown to produce PHB on its own in E. coli under the control of an IPTG-inducible promoter. HPLC was used to identify and quantify the PHB produced. Results can be found on our part's Registry page.

Collaboration

View our Canadian iGEM newsletter and wet-lab work with other iGEM teams on our Collaborations page!

Human Practices Silver

We carefully weighed the effects our project could have in a variety of applications, and considered possible benefits, challenges, and safety concerns . See our Human Practices Silver page for more info!

Works Cited

Hiroe, A., Tsuge, K., Nomura, C.T., Itaya, M. & Tsuge, T. (2012). Rearrangement of Gene Order in the phaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli. Appl. Environ. Microbiol., 78(9): 3177-3184

References

Rose, C., Parker, A., Jefferson, B., & Cartmell, E. (2015). The Characterization of Feces and Urine: A Review of the Literature to Inform Advanced Treatment Technology. Critical Reviews In Environmental Science And Technology, 45(17), 1827-1879. http://dx.doi.org/10.1080/10643389.2014.1000761