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<p>The plasmid with chaperones and the plasmid with substrates have different antibiotic resistance genes to make it possible to select the bacteria’s that expresses both plasmids. </p> | <p>The plasmid with chaperones and the plasmid with substrates have different antibiotic resistance genes to make it possible to select the bacteria’s that expresses both plasmids. </p> | ||
<p>We will induce the expression of the chaperones before we induce the expression of the substrates to make sure that there are chaperones present when the translation of the substrates begins. </p> | <p>We will induce the expression of the chaperones before we induce the expression of the substrates to make sure that there are chaperones present when the translation of the substrates begins. </p> | ||
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+ | Both fusion proteins are fluorescent which will enable us to get an indication of the expression. Further we will use several methods to detect and quantify our proteins. </p> | ||
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The overview of the project is summarized in figure 1 below. As can be seen in the figure, chaperones and conditions will be tested with various experiments, with the purpose to find the best setup for further detailed studies. The detailed studies will involve modeling with systems biology, where the modeling team will give suggestions on what the laboratory team shall do to achieve the desired task. The flow of data between the laboratory and the modeling team will eventually result in the final data, with is the optimized setup for the expression of our peptides.</p> | The overview of the project is summarized in figure 1 below. As can be seen in the figure, chaperones and conditions will be tested with various experiments, with the purpose to find the best setup for further detailed studies. The detailed studies will involve modeling with systems biology, where the modeling team will give suggestions on what the laboratory team shall do to achieve the desired task. The flow of data between the laboratory and the modeling team will eventually result in the final data, with is the optimized setup for the expression of our peptides.</p> | ||
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<img class="content_image" src="https://static.igem.org/mediawiki/2017/5/5c/T--Linkoping_Sweden--Updated_detailed_flow-chart.png" alt="Figure 1. Detaield flow chart of the project."/> | <img class="content_image" src="https://static.igem.org/mediawiki/2017/5/5c/T--Linkoping_Sweden--Updated_detailed_flow-chart.png" alt="Figure 1. Detaield flow chart of the project."/> |
Revision as of 11:56, 27 June 2017