Difference between revisions of "Team:Kyoto/Basic Part"
| Line 6: | Line 6: | ||
<header> | <header> | ||
<ul class="nv"> | <ul class="nv"> | ||
| − | <li><a href="https://2017.igem.org/Team:Kyoto"> | + | <li><a href="https://2017.igem.org/Team:Kyoto"><img src="https://static.igem.org/mediawiki/2017/4/4a/Kyototitle222.png" width="13%"></a></li> |
<li><a href="https://2017.igem.org/team:Kyoto/Project">Project</a> | <li><a href="https://2017.igem.org/team:Kyoto/Project">Project</a> | ||
<ul> | <ul> | ||
<li><a href="https://2017.igem.org/team:Kyoto/Project">Description</a></li> | <li><a href="https://2017.igem.org/team:Kyoto/Project">Description</a></li> | ||
<li><a href="https://2017.igem.org/Team:Kyoto/Results">Results</a></li> | <li><a href="https://2017.igem.org/Team:Kyoto/Results">Results</a></li> | ||
| − | <li><a href="https://2017.igem.org/Team:Kyoto/Demonstrate">Demonstrate</li> | + | <li><a href="https://2017.igem.org/Team:Kyoto/Demonstrate">Demonstrate</a></li> |
| + | <li><a href="https://2017.igem.org/Team:Kyoto/Discussion">Discussion</a></li> | ||
<li><a href="https://2017.igem.org/Team:Kyoto/Basic_Part">Basic parts</a></li> | <li><a href="https://2017.igem.org/Team:Kyoto/Basic_Part">Basic parts</a></li> | ||
<li><a href="https://2017.igem.org/Team:Kyoto/Composite_Part">Composite parts</a></li> | <li><a href="https://2017.igem.org/Team:Kyoto/Composite_Part">Composite parts</a></li> | ||
| Line 22: | Line 23: | ||
</ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Notebook">Notebook</a></li><li><a href="https://2017.igem.org/Team:Kyoto/integrated">Practice</a> | </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Notebook">Notebook</a></li><li><a href="https://2017.igem.org/Team:Kyoto/integrated">Practice</a> | ||
<ul> | <ul> | ||
| − | <li><a href="https://2017.igem.org/Team:Kyoto/Human_Practices">Human Practice</li> | + | <li><a href="https://2017.igem.org/Team:Kyoto/Human_Practices">Human Practice</a></li> |
| − | <li><a href="https://2017.igem.org/Team:Kyoto/Integrated_Practices">Integrated Human Practice</li> | + | <li><a href="https://2017.igem.org/Team:Kyoto/Integrated_Practices">Integrated Human Practice</a></li> |
| − | <li><a href="https://2017.igem.org/Team:Kyoto/Engagement">Public Engagement</li> | + | <li><a href="https://2017.igem.org/Team:Kyoto/Engagement">Public Engagement</a></li> |
| − | <li><a href="https://2017.igem.org/Team:Kyoto/Collaborations">Collaboration</li> | + | <li><a href="https://2017.igem.org/Team:Kyoto/Collaborations">Collaboration</a></li> |
| + | <li><a href="https://2017.igem.org/Team:Kyoto/Discussion_HP">Discussion of HP</a></li> | ||
</ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Team">Team</a> | </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Team">Team</a> | ||
<ul> | <ul> | ||
| − | <li><a href="https://2017.igem.org/Team:Kyoto/Team">Members</li> | + | <li><a href="https://2017.igem.org/Team:Kyoto/Team">Members</a></li> |
| − | <li><a href="https://2017.igem.org/Team:Kyoto/Attributions">Attributions</li> | + | <li><a href="https://2017.igem.org/Team:Kyoto/Attributions">Attributions</a></li> |
</ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Safety">Safety</a></li> | </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
</header> | </header> | ||
| − | |||
<h1>Basic Part</h1> | <h1>Basic Part</h1> | ||
Revision as of 06:43, 30 October 2017
Basic Part
Rev Protein
For this year’s iGEM competition, we have chosen to present Rev protein for the award of the basic part(BBa_K2403000). In synthetic biology, RNA which has a complex structure is frequently designed and used. If such RNA is transferred in Eukaryotic cell, Tap/p15 cannot transfer normal single mRNA to cytoplasm, and the RNA can remain in the nucleus. We introduced Rev protein and RRE (rev response element) to transfer such artificial RNA to the cytoplasm. iGEMers can transport the RNA to the cytoplasm if they bind RRE to any RNA and express Rev protein simultaneously.
How Rev works
Rev protein binds to target sequence, RRE. CRM1, a principal nuclear export factor in cells, is RnaGTP-dependent, binds to Rev protein, and transfers Ribonucleoprotein(RNP) to the extranuclear.
Key advantage
Rev protein makes it possible to transport RNA to the extranuclear which is needed but can’t be normally.
