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<p><h3>Background</h3></p> | <p><h3>Background</h3></p> | ||
<p> | <p> | ||
− | <p style="font-family: roboto;font-size:115%;">Reliable and repeatable measurement is the golden rule of engineering, and so | + | <p style="font-family: roboto;font-size:115%;">Reliable and repeatable measurement is the golden rule of engineering, and so does synthetic biology. However, most of the fluorescent measurement data generated nowadays cannot be compared because it is usually reported in relative unit, but not in absolute unit. In addition, different groups may perform measurement with different protocols, which makes it hard to reproduce. Therefore, iGEM developed a green fluorescent protein (GFP) measurement protocol in order to produce a more reliable, repeatable measurement of GFP. GFP is one of the most commonly used reporter for measurement and easily to be measured in most of laboratories. In the protocol,the unit for fluorescence data is unified so that the results can be compared. The InterLab Study protocol also unifies the measurement procedures and prevents different data processing for the measurement. </font> |
</p> | </p> | ||
− | <p><h3>The Fourth InterLab</h3></p> | + | <p><h3>The Fourth InterLab Study</h3></p> |
− | <p style="font-family: roboto;font-size:115%;">This year, iGEM invited all teams | + | <p style="font-family: roboto;font-size:115%;">This year, iGEM invited all teams to join the fourth InterLab Study. The aim of the study is to find out how close can the numbers be when fluorescence is measured all around the world using the same InterLab protocol and constructs. We registered and measured all the parts according to the instructions.</font> |
</p> | </p> | ||
− | <p><h3> | + | <p><h3>Experiments</h3></p> |
− | <p style="font-family: roboto;font-size:115%;">iGEM provided 8 plasmids for the InterLab Study. Devices 1-6 and positive control have the same reporter gene (GFP), terminator (B0015) and backbone (pSB1C3). However, devices 1-3 share the same RBS (B0034), while devices 4-6 share another modified RBS called bicistronic device (BCD2). Different promoters are also used in different | + | <p style="font-family: roboto;font-size:115%;">iGEM headquarter provided 8 plasmids for the InterLab Study. Devices 1-6 and positive control have the same reporter gene (GFP), terminator (B0015) and backbone (pSB1C3). However, devices 1-3 share the same RBS (B0034), while devices 4-6 share another modified RBS called bicistronic device (BCD2). Different promoters are also used in different plasmids. According to the strength of promoter described by iGEM2006_Berkeley team, device 1 should have the strongest fluorescence and device 3 should have the weakest among devices 1-3, while Device 4 should have the strongest fluorescence and device 6 should have the weakest among devices 4-6.</font> |
</p> | </p> | ||
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<p> | <p> | ||
− | <h3>What is Bicistronic Device (BCD)?</h3> | + | <h3>What is a Bicistronic Device (BCD)?</h3> |
</p> | </p> | ||
Revision as of 08:49, 30 October 2017