|
|
| Line 180: |
Line 180: |
| | <div class="col-md-12"> | | <div class="col-md-12"> |
| | <div class="block"> | | <div class="block"> |
| − | <h2>COMMON Page</h2> | + | <h2>FBPA for Synechococcus sp. PCC 7002</h2> |
| | </div> | | </div> |
| | </div> | | </div> |
| Line 190: |
Line 190: |
| | <div class="row"> | | <div class="row"> |
| | <div class="col-md-12"> | | <div class="col-md-12"> |
| − | <div class="nj-box3">
| |
| − | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/0/03/T-worldshaper-XSHS-00004.jpeg" />
| |
| − | </div>
| |
| | <div class="nj-box4"> | | <div class="nj-box4"> |
| | <p> | | <p> |
| − | Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quas sit expedita, iusto repellendus cumque, officia architecto consequatur illo fuga eum sed ut autem eos voluptas. Nemo, a, rem! Atque quisquam aperiam eaque tenetur autem, soluta itaque omnis. Minus nesciunt, sint, animi illum quo ab voluptate esse delectus unde maiores iure, quasi a suscipit ipsam aliquid voluptatem. Perspiciatis eveniet, pariatur illum aut cum dolor neque consequatur error aliquid facilis in quasi temporibus assumenda tempore, doloremque autem saepe enim nihil. Voluptates asperiores ullam voluptate quas similique ratione quia hic, eum distinctio laboriosam, consectetur tempora voluptatibus optio natus cumque est necessitatibus dolore alias. | + | BBa_K2513032 |
| | </p> | | </p> |
| | + | <p>FBPA for Synechococcus sp. PCC 7002</p> |
| | + | <div class="nj-box3"> |
| | + | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/0/03/T-worldshaper-XSHS-00004.jpeg" /> |
| | + | </div> |
| | + | <p>This is an improved version of BBa_K1122702 which encodes a protein capable of binding ferric iron. We optimized its coding sequence according to the favorite codon usage frequency of Synechococcus sp. PCC 7002. Further we added NcoI and BamHI sites at 5 prime end, and added HindIII and BglII sites at 3 prime end. It could be easily fused with INP or SomA which are well verified proteins that used in surface-display in E.coli and Synechococcus sp. PCC 7002, so that the FBPA could be also expressed at the surface of E.coli or Synechococcus sp. PCC 7002.</p> |
| | <p> | | <p> |
| − | Lorem ipsum dolor sit amet, consectetur adipisicing elit. Veritatis expedita repellendus laboriosam aliquid. Neque doloribus ea, id reprehenderit alias saepe debitis eligendi molestias odit, nesciunt rem. Dolorem saepe, provident dolore nesciunt laudantium nostrum enim natus veritatis harum maxime et iure ratione, nulla. Minus excepturi commodi tempore voluptate. Blanditiis similique dolor asperiores ex excepturi perspiciatis, dolores id esse. Voluptate beatae nesciunt cum esse ratione officiis necessitatibus blanditiis ea, laboriosam fugit vero maxime? Voluptatum illo dolorum autem pariatur quisquam. Voluptates soluta culpa necessitatibus veritatis tempora incidunt doloribus placeat repellat et facilis eum sapiente fugit numquam aut, laboriosam aspernatur, esse, magnam excepturi repudiandae amet voluptas nulla quidem. Veritatis nisi consequuntur saepe qui quisquam dignissimos assumenda, iusto odio. Dignissimos reprehenderit esse iusto cupiditate nisi enim, animi similique itaque, perspiciatis error qui. Aperiam, architecto provident. | + | FBPA is from Neisseria gonorrhoeae. The whole parts is synthesized by GenScript Corporation. |
| | </p> | | </p> |
| | + | <p>Because of the special bias of codon usage of Synechococcus sp. PCC 7002, it was necessary to do a codon optimization of FBPA. And in order to express this ferric ion binding protein at the surface of Synechococcus sp. PCC 7002, this part was designed to be fused with other outer membrane proteins like INP or SomA with or without stop codon and with unique restriction sites of BamHI and HindIII at the two sides.</p> |
| | </div> | | </div> |
| | </div> | | </div> |
