Line 60: | Line 60: | ||
<b>UCCKE:</b><br> | <b>UCCKE:</b><br> | ||
Uric acid assay | Uric acid assay | ||
+ | <br> | ||
The protocol is designed and provided by us. | The protocol is designed and provided by us. | ||
+ | <br> | ||
+ | <br> | ||
Materials: | Materials: | ||
+ | <br> | ||
Project300 part in DH5a E coli | Project300 part in DH5a E coli | ||
+ | <br> | ||
LB+ Chloramphenicol | LB+ Chloramphenicol | ||
− | Uric acid | + | <br>Uric acid |
− | 96 well plate+ ClarioSTAR plate reader | + | <br>96 well plate+ ClarioSTAR plate reader |
− | Transform 300 plasmid into Dh5a E coli cell | + | <br> |
− | Pick a clone for the 300 plasmid into 5ml LB with Chloramphenicol | + | 1.Transform 300 plasmid into Dh5a E coli cell |
− | Prepare saturated uric acid solution with LB solution and perform 1:1 serial dilution to make 5 different concentrations of uric acid in LB. | + | <br>2.Pick a clone for the 300 plasmid into 5ml LB with Chloramphenicol |
− | Inoculate 2ml of LB into 250 ml of LB with Chloramphenicol and varing concentration of uric acid in 37C overnight. | + | <br>3.Prepare saturated uric acid solution with LB solution and perform 1:1 serial dilution to make 5 different concentrations of uric acid in LB. |
− | Measure the fluorescence of GFP(absoprtion:583nm emittion:607nm) by ClarioSTAR plate reader. | + | <br>4.Inoculate 2ml of LB into 250 ml of LB with Chloramphenicol and varing concentration of uric acid in 37C overnight. |
+ | <br>5.Measure the fluorescence of GFP(absoprtion:583nm emittion:607nm) of 200μl of biobrick by ClarioSTAR plate reader. | ||
<br><br> | <br><br> | ||
<b>HKUST:</b><br> | <b>HKUST:</b><br> |
Revision as of 11:42, 30 October 2017