Difference between revisions of "Team:Oxford/Protocols"

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<p> We wanted to have a page of our wiki where we summarised the steps that we took from designing our parts through to sending them to iGEM to add to the registry, it is the sort of page we would have found useful when we first started so we had a good overview.
 
<p> We wanted to have a page of our wiki where we summarised the steps that we took from designing our parts through to sending them to iGEM to add to the registry, it is the sort of page we would have found useful when we first started so we had a good overview.
  
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Revision as of 12:35, 30 October 2017


Routine Cloning

We wanted to have a page of our wiki where we summarised the steps that we took from designing our parts through to sending them to iGEM to add to the registry, it is the sort of page we would have found useful when we first started so we had a good overview.

Create DNA in Snapgene
Order DNA from IDT
PCR from G-Blocks
Run a Gel of the PCR
Digest and Ligate
Transform and Plate
Inoculate - Placeholder Image
Miniprep
Test Digest and Sequence
Send DNA to iGEM