Difference between revisions of "Team:Oxford/Protocols"

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<p> We wanted to have a page of our wiki where we summarised the steps that we took from designing our parts all the way through to sending them to iGEM to add to the registry. It is the sort of overview we would have found useful when we first started, and we hope it will help other teams. <p>
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<p> This page includes all our protocols that we made we were cloning initially, as well as being a repository for protocols we made for experiments that did or didn't work to characterise our parts. Although a lot of them are simple protocols, and probably do not vary much from other teams', we have left them as we used them, so that other teams can get an indication of the sort of things we feel are important to include when you make protocols for your team. We used Benchling to write them, and it meant that demonstrators only had to explain things to one member of our team, rather than 12 different times!  <p>
  
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<h2> Protocols for Steps </h2>  
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<h2> Protocols for Initial Cloning </h2>
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<p> We wanted part of this page to summarise the steps that we took from designing our parts all the way through to sending them to iGEM to add to the registry. It is the sort of overview we would have found useful when we first started, and we hope it will help other teams. </p>  
  
 
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Revision as of 12:43, 30 October 2017


Protocols

This page includes all our protocols that we made we were cloning initially, as well as being a repository for protocols we made for experiments that did or didn't work to characterise our parts. Although a lot of them are simple protocols, and probably do not vary much from other teams', we have left them as we used them, so that other teams can get an indication of the sort of things we feel are important to include when you make protocols for your team. We used Benchling to write them, and it meant that demonstrators only had to explain things to one member of our team, rather than 12 different times!

Protocols for Initial Cloning

We wanted part of this page to summarise the steps that we took from designing our parts all the way through to sending them to iGEM to add to the registry. It is the sort of overview we would have found useful when we first started, and we hope it will help other teams.

Create DNA in Snapgene
Order DNA from IDT
PCR from G-Blocks
Run a Gel of the PCR
Digest and Ligate
Transform and Plate
Inoculate - Placeholder Image
Miniprep
Test Digest and Sequence
Send DNA to iGEM