Difference between revisions of "Team:WHU-China/Composite Part"

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   <h2>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Brick2 <i>RdhANP</i> combined with a biosensor (BBa_K2462007)</h2>
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   <h2>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Brick2 RdhANP combined with a biosensor (BBa_K2462007)</h2>
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<h3>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As we designed our project, we have wondered if there exists any regulation mechanism to make the expression of <i>RdhANP</i> more precisely. We searched the information and find a biosensor created by team Peking-China in 2013. This biosensor can function when it runs into substances analogous to salicylic acid. And some of the substances they tested can serve as the substrates of <i>RdhANP</i> , like 2,4,6-trichloro-phenol. We then linked this sensor(BBa_J61051) with <i>RdhANP</i> and the enzyme is supposed to express when it's substrates are available.  
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<h3>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As we designed our project, we have wondered if there exists any regulation mechanism to make the expression of RdhANP more precisely. We searched the information and find a biosensor created by team Peking-China in 2013. This biosensor can function when it runs into substances analogous to salicylic acid. And some of the substances they tested can serve as the substrates of RdhANP , like 2,4,6-trichloro-phenol. We then linked this sensor(BBa_J61051) with RdhANP and the enzyme is supposed to express when it's substrates are available.  
 
The biosensor made by Peking-China: <a href="http://parts.igem.org/Part:BBa_J61051">http://parts.igem.org/Part:BBa_J61051</a>
 
The biosensor made by Peking-China: <a href="http://parts.igem.org/Part:BBa_J61051">http://parts.igem.org/Part:BBa_J61051</a>
 
</h3>
 
</h3>

Revision as of 12:55, 30 October 2017

Composite Parts

         Brick2 RdhANP combined with a biosensor (BBa_K2462007)

         As we designed our project, we have wondered if there exists any regulation mechanism to make the expression of RdhANP more precisely. We searched the information and find a biosensor created by team Peking-China in 2013. This biosensor can function when it runs into substances analogous to salicylic acid. And some of the substances they tested can serve as the substrates of RdhANP , like 2,4,6-trichloro-phenol. We then linked this sensor(BBa_J61051) with RdhANP and the enzyme is supposed to express when it's substrates are available. The biosensor made by Peking-China: http://parts.igem.org/Part:BBa_J61051

         Brick7 Combination of promoters in brick4 (BBa_K2462004) with RBS（BBa_B0030） (BBa_K2462006)

         We improve a previous part(BBa_B0030) by combining it with the promoter used in Bacillus megaterium. As the strength of RBS(BBa_B0030) has already been tested and the promoters of these two composite bricks are suitable for Bacillus megaterium, it’s quite convenient for other teams to use.